Huang Dongmei, Lan Wei, Li Danjing, Deng Ban, Lin Wenfang, Ren Yujun, Miao Ying
Center for Molecular Cell and Systems Biology, Fujian Provincial Key Laboratory of Haixia Applied Plant Systems Biology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.
Front Plant Sci. 2018 Oct 19;9:1503. doi: 10.3389/fpls.2018.01503. eCollection 2018.
Single-stranded DNA-binding proteins (SSBs) are assumed to involve in DNA replication, DNA repairmen, and gene transcription. Here, we provide the direct evidence on the functionality of an SSB, WHIRLY1, by using loss- or gain-of-function lines. We show that WHIRLY1 binding to the promoter of represses the enrichment of H3K4me3, but enhances the enrichment of H3K9ac at the region contained WHIRLY1-binding sequences and TATA box or the translation start region of , coincided with a recruitment of RNAPII. ChIP assays confirm that WHIRLY1 inhibits H3K4me3 enrichment at the preinitiation complex formation stage, while promotes H3K9ac enrichment and RNAPII recruitment at the elongation stage, consequently affecting the transcription of . These results further explore the molecular actions underlying SSB-mediated gene transcription through epigenetic regulation in plant senescence.
单链DNA结合蛋白（SSBs）被认为参与DNA复制、DNA修复和基因转录。在此，我们通过使用功能缺失或功能获得系，提供了关于一种单链DNA结合蛋白WHIRLY1功能的直接证据。我们表明，WHIRLY1与[具体基因名称]启动子的结合抑制了H3K4me3的富集，但增强了在包含WHIRLY1结合序列和TATA盒或[具体基因名称]翻译起始区域的区域中H3K9ac的富集，这与RNA聚合酶II（RNAPII）的募集相吻合。染色质免疫沉淀（ChIP）分析证实，WHIRLY1在起始前复合物形成阶段抑制H3K4me3富集，而在延伸阶段促进H3K9ac富集和RNAPII募集，从而影响[具体基因名称]的转录。这些结果进一步探索了在植物衰老过程中，单链DNA结合蛋白通过表观遗传调控介导基因转录的分子作用。
