高效液相色谱法测定不同产地及品种石韦中绿原酸和芒果苷的含量
[Determination of chlorogenic acid and mangiferin in Folium Pyrrosiae from different habitats and species by HPLC].
作者信息
Li Kaitong, Zhang Yixuan, Cao Yang, Shi Yue
机构信息
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2075-8.
An HPLC method for simultaneous determination of chlorogenic acid and mangiferin in original medicinal materials and decoction pieces of Pyrrosiae Folium was developed. The assay was performed on a Diamonsil C18 (4.6 mm x 250 mm, 5 microm) column eluted with a mobile phase consisted of acetonitrile and 0.5% phosphoric acid solution in gradient elution at a flow rate of 1.0 mL x min(-1). The column temperature was set at 25 degrees C. The detection wavelength was 320 nm. The results showed that The linear ranges of chlorogenic acid and mangiferin were 5.2-130 mg x L(-1) (r = 0.9999) and 1.2-18 microg x mL(-1) (r = 0.9999), and the average recoveries (n=6) were 97.9% (RSD 1.9%) and 99.6% (RSD 2.9%), respectively. The method was simple, reproducible and valid. It can be used for quality evaluation and control of original medicinal materials and decoction pieces of Pyrrosiae Folium.
建立了一种同时测定石韦原药材及饮片绿原酸和芒果苷含量的高效液相色谱法。采用Diamonsil C18柱（4.6 mm×250 mm，5μm），以乙腈和0.5%磷酸溶液为流动相进行梯度洗脱，流速为1.0 mL·min-1。柱温设定为25℃，检测波长为320 nm。结果表明，绿原酸和芒果苷的线性范围分别为5.2 - 130 mg·L-1（r = 0.9999）和1.2 - 18μg·mL-1（r = 0.9999），平均回收率（n = 6）分别为97.9%（RSD 1.9%）和99.6%（RSD 2.9%）。该方法简便、重现性好、结果可靠，可用于石韦原药材及饮片的质量评价与控制。
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