Yu Shengxian, Yuan Qingjun, Yang Bin, Zhang Chunxia, Chen Chengyu
Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2129-32.
population genetic study of Magnolia officinalis and M. officinalis var. biloba was performed in this paper. The objectives of this study are to provide baseline data of molecular pharmaceutics for quality control of Cortex Magnoliae Officinalis.
A total of 15 populations of M. officinalis and M. officinalis var. biloba were collected. PCR amplification and sequencing were performed with two chloroplast intergenic spacers psbA-trnH and trnL-trnF. Chloroplast haplotype frequencies were calculated, genetic diversity and genetic structure were estimated by using the program HAPLONST, and a haplotype network depicting the mutational relationships among distinct haplotypes was drawn following the principle of parsimony by TCS version 1.13.
The differentiation of haplotype frequencies between M. officinalis and M. officiunalis var. biloba was significant although there was no private haplotypes of themselves.
There was certain genetic divergence between M. officinalis and M. offcinalis var. biloba although the monophyletic clade of themselves was not still formed.
对厚朴和凹叶厚朴进行群体遗传学研究。本研究的目的是为厚朴药材质量控制提供分子药学的基础数据。
共收集了15个厚朴和凹叶厚朴群体。采用两个叶绿体基因间隔区psbA-trnH和trnL-trnF进行PCR扩增和测序。计算叶绿体单倍型频率，使用HAPLONST程序估计遗传多样性和遗传结构，并根据简约原则，用TCS 1.13版本绘制描述不同单倍型间突变关系的单倍型网络图。
厚朴和凹叶厚朴的单倍型频率存在显著差异，尽管它们没有各自特有的单倍型。
厚朴和凹叶厚朴之间存在一定的遗传分化，尽管尚未形成各自的单系分支。
