Zhao Yu-Yang, Yang Jian, Zha Liang-Ping, Xie Dong-Mei, Huang Lu-Qi, Yuan Yuan
State Key Laboratory of Dao-di Herbs Breeding Base, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China.
Zhongguo Zhong Yao Za Zhi. 2016 Feb;41(4):648-653. doi: 10.4268/cjcmm20160417.
The study used use bimolecular marking methods to evaluate the lignans of Magnolia officinalis and M. officinalis var. biloba. First, we compare the chemical constituents between M. officinalis and M. officinalis var.biloba. There were significant differences in concentration of magnolignan I between leaves of these two varieties. Then we further select the p-hydroxyphenyl lignin to mining the key enzyme genes of biosynthesis from Magnolia transcriptome, and screened an encoding cinnamyl alcohol dehydrogease gene as the candidate marker of bimolecular marking methods of Magnolia quality by comparing of the expression level and structure variation in homologous gene between M. officinalis and M. officinalis var.biloba. The established method provides the technical support for bimolecular marking methods of Magnolia quality evaluation.
该研究采用双分子标记方法对厚朴和凹叶厚朴的木脂素进行评估。首先，我们比较了厚朴和凹叶厚朴的化学成分。这两个品种叶片中厚朴脂素I的浓度存在显著差异。然后我们进一步选择对羟基苯基木脂素，从厚朴转录组中挖掘生物合成的关键酶基因，并通过比较厚朴和凹叶厚朴同源基因的表达水平和结构变异，筛选出一个编码肉桂醇脱氢酶的基因作为厚朴品质双分子标记方法的候选标记。所建立的方法为厚朴品质评价的双分子标记方法提供了技术支持。
