[高效液相色谱法同时测定血府逐瘀颗粒中多组分在不同紫外波长下的含量]

[Simultaneous determination of multicomponent in Xuefuzhuyu granule with different UV-wavelength by HPLC].

作者信息

Liu Zhenghui, Ye Tingxiang, Zhao Linlin, Cheng Yi, Luo Guoan

机构信息

Tianjin High-Standard Quality Testing Lab., Tianjin 300081, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2010 Aug;35(16):2157-60.

PMID:21046752

Abstract

OBJECTIVE

To establish the method for the quality control of Xuefuzhuyu granule.

METHOD

The content of Semen Armeniacae Amarum PE, Hydroxysafflor yellow A, paeoniflorin and ferulic acid in Xuefuzhuyu granule were determined by HPLC under multi-wavelength of 210, 403, 230, 316 nm.

RESULT

The calibration curve of Semen Armeniacae Amarum PE. hydroxysafflor yellow A, paeoniflorin and ferulic acid were linear within 0.1154-0.9232 (r = 0.9998), 0.0219-0.1754 (r = 0.9994), 0.3952-3.1616 (r = 0.9995), and 0.0426-0.3408 microg (r = 0.9998), respectively. The average recoveries were 101.78% (RSD was 1.7%), 99.60% (RSD was 2.9%), 98.90% (RSD was 2.0%), and 100.31% (RSD was 1.8%), respectively.

CONCLUSION

The method of quantification is accurate, rapid and reliable for the quality control of Xuefuzhuyu granule.

摘要

目的

建立血府逐瘀颗粒的质量控制方法。

方法

采用高效液相色谱法，在210、403、230、316nm多波长下测定血府逐瘀颗粒中苦杏仁苷、羟基红花黄色素A、芍药苷和阿魏酸的含量。

结果

苦杏仁苷、羟基红花黄色素A、芍药苷和阿魏酸的校准曲线分别在0.1154 - 0.9232（r = 0.9998）、0.0219 - 0.1754（r = 0.9994）、0.3952 - 3.1616（r = 0.9995）和0.0426 - 0.3408μg（r = 0.9998）范围内呈线性关系。平均回收率分别为101.78%（相对标准偏差为1.7%）、99.60%（相对标准偏差为2.9%）、98.90%（相对标准偏差为2.0%）和100.31%（相对标准偏差为1.8%）。