Takayama T K, Miller C, Szabo G
Department of Surgery, University of Massachusetts Medical Center, Worcester 01655.
Arch Surg. 1990 Jan;125(1):29-35. doi: 10.1001/archsurg.1990.01410130031004.
The level of tumor necrosis factor alpha (TNF alpha), a monokine implicated in mediating septic shock, is elevated in the blood of some patients with sepsis. Monocytes from 11 trauma patients and 11 burn patients were suboptimally stimulated with interferon gamma and muramyl dipeptide, an analogue of bacterial wall products. The patients with sepsis showed significantly greater total TNF alpha levels (secreted in combination with cell-associated) 3 days before septic episodes, as compared with normal controls (32.38 to 2231.76 ng/10(6) monocytes per milliliter, median = 121.03 ng/10(6) monocytes per milliliter; normal control: 0.00 to 18.20 ng/10(6) monocytes per milliliter, median = 5.93 ng/10(6) monocytes per milliliter). Increases in patients' total monocyte TNF alpha levels greater than 30 ng/10(6) monocytes per milliliter correlated with septic episodes. In patients with sepsis, the total monocyte TNF alpha levels were increased despite a concomitant increase in their prostaglandin E2 levels in both stimulated (interferon gamma plus muramyl dipeptide) and unstimulated in vitro assays (9 patients: stimulated prostaglandin E2 range, 30.1 to 123.6 ng/10(6) monocytes per milliliter). Massively elevated monocyte TNF alpha and prostaglandin E2 production occurred simultaneously in patients with sepsis.
肿瘤坏死因子α（TNFα）是一种与介导脓毒症休克有关的单核因子，在一些脓毒症患者的血液中水平升高。对11名创伤患者和11名烧伤患者的单核细胞用γ干扰素和胞壁酰二肽（一种细菌壁产物类似物）进行次优刺激。与正常对照组相比，脓毒症患者在脓毒症发作前3天的总TNFα水平（分泌型与细胞相关型之和）显著更高（32.38至2231.76纳克/每毫升10⁶单核细胞，中位数 = 121.03纳克/每毫升10⁶单核细胞；正常对照组：0.00至18.20纳克/每毫升10⁶单核细胞，中位数 = 5.93纳克/每毫升10⁶单核细胞）。患者总单核细胞TNFα水平每毫升每10⁶单核细胞升高超过30纳克与脓毒症发作相关。在脓毒症患者中，无论在体外刺激试验（γ干扰素加胞壁酰二肽）还是未刺激试验中，尽管其前列腺素E2水平同时升高，但总单核细胞TNFα水平仍升高（9名患者：刺激后前列腺素E2范围为每毫升每10⁶单核细胞30.1至123.6纳克）。脓毒症患者中单核细胞TNFα和前列腺素E2的大量升高同时出现。
