Miller-Graziano C L, Szabo G, Griffey K, Mehta B, Kodys K, Catalano D
Department of Surgery, University of Massachusetts Medical Center, Worcester 01655.
J Clin Immunol. 1991 Mar;11(2):95-102. doi: 10.1007/BF00917745.
We previously reported that increased production of prostaglandin E2 by monocytes is a pivotal mechanism in posttrauma immunopathology. Here we characterize monocyte levels of transforming growth factor beta and examine the effects of elevated transforming growth factor beta on prostaglandin E2 release by patients' monocytes. Trauma patients' and normals' monocyte supernates (+/- stimulation with muramyl dipeptide) were acid treated and assayed for transforming growth factor beta using the mink lung-cell bioassay. Alternatively, human transforming growth factor beta was added to patients' and normals' monocytes and prostaglandin E2 production assayed. Significantly elevated transforming growth factor beta levels (median = 181.7 pmol/10(6) monocytes) were detected in immunosuppressed patients' monocytes but not immunocompetent trauma patients' (median = 32.0 pM) or normals' (median = 20.4 pM) monocytes. Adding transforming growth factor beta to monocytes resulted in a significant elevation of prostaglandin E2 levels. Elevated monocyte transforming growth factor beta levels in trauma patients could be both suppressing T-lymphocyte functions and maintaining elevated monocyte prostaglandin E2 synthesis.
我们之前报道过，单核细胞中前列腺素E2产量增加是创伤后免疫病理学的关键机制。在此，我们对转化生长因子β的单核细胞水平进行了表征，并研究了转化生长因子β升高对患者单核细胞释放前列腺素E2的影响。对创伤患者和正常人的单核细胞上清液（用胞壁酰二肽刺激或不刺激）进行酸处理，并使用貂肺细胞生物测定法检测转化生长因子β。或者，将人转化生长因子β添加到患者和正常人的单核细胞中，并检测前列腺素E2的产生。在免疫抑制患者的单核细胞中检测到转化生长因子β水平显著升高（中位数 = 181.7 pmol/10(6) 单核细胞），但在免疫功能正常的创伤患者（中位数 = 32.0 pM）或正常人（中位数 = 20.4 pM）的单核细胞中未检测到。向单核细胞中添加转化生长因子β会导致前列腺素E2水平显著升高。创伤患者单核细胞中转化生长因子β水平升高可能既抑制T淋巴细胞功能，又维持单核细胞前列腺素E2合成升高。
