Vader H L, Geuskens L M, Vink C L
Clin Chim Acta. 1977 Oct 15;80(2):361-72. doi: 10.1016/0009-8981(77)90045-6.
In this study, using radioimmunoassay techniques, we found that ions at concentrations in the order of 0.1 molar influence the antigen-antibody complex formation. The angiotensin I/anti-angiotensin I reaction was studied in detail. Particularly bivalent cations and anions with a strong chaotropic effect (SCN-, I- and ClO4-) were found to influence strongly the specific immunological reaction. However, NO3- had also a remarkably strong influence. We found that the equilibrium constant, rather than the number of binding sites of the antibody, is influenced by the ions. It should be borne in mind that relatively high concentrations of electrolyte (as compared with the concentrations of antigen and antibody) show this effect. Consequently, this effect is of less practical importance for routine radioimmunoassay than is, for example, the effect of pH. However, this phenomenon shows that the radioimmunoassay technique might be valuable not only for quantization of very low hormone concentrations in biological fluids, but has also important potential applications in physical and protein chemistry. Particularly, the high sensitivity of this technique and the possibility of studying a homogeneous reaction system might give it advantages over other techniques.
在本研究中，我们采用放射免疫分析技术发现，浓度在0.1摩尔数量级的离子会影响抗原 - 抗体复合物的形成。我们对血管紧张素I/抗血管紧张素I反应进行了详细研究。特别发现，具有强离液序列高变效应的二价阳离子和阴离子（SCN-、I-和ClO4-）会强烈影响特异性免疫反应。然而，NO3-也有显著的强烈影响。我们发现，受离子影响的是平衡常数，而非抗体的结合位点数。应当记住，相对高浓度的电解质（与抗原和抗体的浓度相比）会表现出这种效应。因此，与例如pH的影响相比，这种效应在常规放射免疫分析中的实际重要性较小。然而，这一现象表明，放射免疫分析技术不仅可能对生物体液中极低激素浓度的定量有价值，而且在物理化学和蛋白质化学中也有重要的潜在应用。特别是，该技术的高灵敏度以及研究均相反应体系的可能性可能使其比其他技术更具优势。
