Department of Veterinary Public Health and Preventive Medicine, University of Ibadan, Ibadan, Nigeria.
J Clin Microbiol. 2011 Jan;49(1):220-6. doi: 10.1128/JCM.01241-10. Epub 2010 Nov 3.
We ran a comparative analysis of all patients for whom a positive culture of Mycobacterium tuberculosis complex was available between April 2004 and October 2005 and whose HIV serology results were known, with spoligotyping results (n = 163) split into 49 HIV-positive patients and 114 HIV-negative patients. Spoligotype international type 373 (SIT373) (T1 lineage), which was highly prevalent among the HIV(+) patients, was totally absent from the HIV(-) population, suggesting that we had a specific clone affecting nearly 1/3 of all HIV-tuberculosis (TB)-coinfected patients. Among the LAM10-CAM sublineage strains, we had only a single strain of SIT403 among HIV(-) patients (0.88%), as opposed to 12.25% of the HIV(+) population (χ(2) = 10.77; P < 0.01), indicating a strong association between the strain and the HIV(+) population. The LAM10-CAM lineage spoligotype SIT61 was prevalent among the 2 subsets (37.72% in HIV(-) versus 12.24% in HIV(+) populations), though, with a significant difference between the 2 groups (χ(2) = 10.53; P < 0.01). However, there was no significant difference for SIT53 (T1 lineage) in the 2 subsets: 6.14 versus 8.2% (χ(2) = 0.22; P > 0.05). A total of 7/49, or 14.3%, other SITs among HIV(+) patients were not found among the HIV(-) patients. When added to the most prevalent SIT among HIV(+) patients (SIT373; n = 16), 23/49, or 47%, isolates among HIV-TB-coinfected patients were unique. We conclude that further studies should be carried out to investigate the evolution of these genotypes and others in the emergence of multidrug resistance and control of tuberculosis in Nigeria.
我们对 2004 年 4 月至 2005 年 10 月间所有分枝杆菌复合群阳性培养患者进行了对比分析，这些患者的 HIV 血清学结果已知， spoligotyping 结果（n=163）分为 49 例 HIV 阳性患者和 114 例 HIV 阴性患者。在 HIV 阳性患者中高度流行的 spoligotype 国际型 373（SIT373）（T1 谱系）在 HIV 阴性人群中完全不存在，这表明我们有一种特定的克隆株影响了近三分之一的所有 HIV-结核（TB）合并感染患者。在 LAM10-CAM 亚谱系菌株中，我们仅在 HIV 阴性患者中发现了一种 SIT403 菌株（0.88%），而 HIV 阳性患者中则为 12.25%（χ2=10.77；P<0.01），表明该菌株与 HIV 阳性人群之间存在很强的关联。LAM10-CAM 谱系 spoligotype SIT61 在两个亚组中都很流行（HIV 阴性组为 37.72%，HIV 阳性组为 12.24%），但两组之间有显著差异（χ2=10.53；P<0.01）。然而，在两个亚组中，SIT53（T1 谱系）没有显著差异：6.14%比 8.2%（χ2=0.22；P>0.05）。在 HIV 阳性患者中，有 7/49，或 14.3%，其他 SIT 未在 HIV 阴性患者中发现。当加上 HIV 阳性患者中最流行的 SIT（SIT373；n=16）时，HIV-TB 合并感染患者中有 23/49，或 47%，是独特的。我们得出结论，应进一步开展研究，以调查这些基因型以及尼日利亚耐多药结核病和结核病控制中其他基因型的演变。
