Genomic Variation Laboratory, Department of Animal Science, University of California, Davis, CA 95616, USA.
Heredity (Edinb). 2011 Jun;106(6):920-6. doi: 10.1038/hdy.2010.137. Epub 2010 Nov 3.
Whirling disease, caused by the pathogen Myxobolus cerebralis, leads to skeletal deformation, neurological impairment and under certain conditions, mortality of juvenile salmonid fishes. The disease has impacted the propagation and survival of many salmonid species over six continents, with particularly negative consequences for rainbow trout. To assess the genetic basis of whirling disease resistance in rainbow trout, genome-wide mapping was initiated using a large outbred F(2) rainbow trout family (n=480) and results were confirmed in three additional outbred F(2) families (n=96 per family). A single quantitative trait locus (QTL) region on chromosome Omy9 was identified in the large mapping family and confirmed in all additional families. This region explains 50-86% of the phenotypic variance across families. Therefore, these data establish that a single QTL region is capable of explaining a large percentage of the phenotypic variance contributing to whirling disease resistance. This is the first genetic region discovered that contributes directly to the whirling disease phenotype and the finding moves the field closer to a mechanistic understanding of resistance to this important disease of salmonid fish.
旋转病，由病原体 Myxobolus cerebralis 引起，导致幼鲑鱼类骨骼变形、神经损伤，在某些情况下导致死亡。这种疾病已经影响了六大洲许多鲑鱼物种的繁殖和生存，对虹鳟鱼的影响尤其严重。为了评估虹鳟鱼对旋转病的遗传基础，使用一个大型杂交 F(2)虹鳟鱼家系（n=480）进行了全基因组图谱绘制，结果在另外三个杂交 F(2)家系中得到了证实（每个家系 n=96）。在大型图谱家系中鉴定到一个位于 Omy9 染色体上的单一数量性状位点（QTL）区域，并在所有其他家系中得到了证实。该区域解释了家系间 50-86%的表型方差。因此，这些数据表明，单一的 QTL 区域能够解释导致旋转病抗性的表型方差的很大一部分。这是第一个直接导致旋转病表型的遗传区域，这一发现使该领域更接近于对鲑鱼鱼类这一重要疾病的抗性的机制理解。
