Vetvicka Vaclav, Vancikova Zuzana
University of Louisville, Department of Pathology, KY, USA.
Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub. 2010 Sep;154(3):235-8. doi: 10.5507/bp.2010.035.
β-Glucans are well-established immunomodulators. Recently, glucans have been found to influence stress-related immunosuppression.
The aim of this study was to compare the effects of four different types of β-glucans on immune reactions suppressed by cold- or restrain-induced stress.
Mice were subjected to restraint and cold stress for various time intervals. The ability of individual glucans to overcome stress-related changes was evaluated after 14 days of feeding.
First, we showed that cold stress caused 38% decrease in phagocytic activity. While all glucans showed some ability to inhibit stress-related inhibition, only glucan #300 was able to return the phagocytosis to a normal level. In the control group, feeding with glucans did not alter the level of corticosterone. On the other hand, both types of stress resulted in a significant increase in corticosterone which was blocked to some extent by feeding with glucan. Both types of stress reduced IL-6 secretion but only glucan #300 managed to keep IL-6 secretion above control levels. The same results were obtained in the case of IL-12.
From our data, we can conclude that, even when all orally-administered glucans helped to restore the stress-related decrease in immune reaction, the level of activity varied widely among individual glucans. In addition, the results suggest that glucans might work via inhibition of corticosterone levels and/or stimulation of cytokine production.
β-葡聚糖是公认的免疫调节剂。最近，已发现葡聚糖会影响与应激相关的免疫抑制。
本研究旨在比较四种不同类型的β-葡聚糖对由寒冷或束缚诱导的应激所抑制的免疫反应的影响。
对小鼠进行不同时间间隔的束缚和寒冷应激。在喂食14天后评估每种葡聚糖克服与应激相关变化的能力。
首先，我们发现寒冷应激导致吞噬活性降低38%。虽然所有葡聚糖都表现出一定抑制与应激相关抑制的能力，但只有葡聚糖#300能够使吞噬作用恢复到正常水平。在对照组中，喂食葡聚糖并未改变皮质酮水平。另一方面，两种应激类型均导致皮质酮显著增加，而喂食葡聚糖在一定程度上可阻止这种增加。两种应激类型均降低了IL-6的分泌，但只有葡聚糖#300设法使IL-6分泌保持在对照水平以上。IL-12的情况也得到了相同结果。
从我们的数据可以得出结论，即使所有口服葡聚糖都有助于恢复与应激相关的免疫反应降低，但各葡聚糖之间的活性水平差异很大。此外，结果表明葡聚糖可能通过抑制皮质酮水平和/或刺激细胞因子产生而起作用。
