Kuhl Heiner, Sarropoulou Elena, Tine Mbaye, Kotoulas Georgios, Magoulas Antonios, Reinhardt Richard
Max Planck Institute for Molecular Genetics, htpt group, Ihnestr. 63, 14195 Berlin, Germany.
J Biomed Biotechnol. 2011;2011:329025. doi: 10.1155/2011/329025. Epub 2010 Oct 13.
This study presents the first comparative BAC map of the gilthead sea bream (Sparus aurata), a highly valuated marine aquaculture fish species in the Mediterranean. High-throughput end sequencing of a BAC library yielded 92,468 reads (60.6 Mbp). Comparative mapping was achieved by anchoring BAC end sequences to the three-spined stickleback (Gasterosteus aculeatus) genome. BACs that were consistently ordered along the stickleback chromosomes accounted for 14,265 clones. A fraction of 5,249 BACs constituted a minimal tiling path that covers 73.5% of the stickleback chromosomes and 70.2% of the genes that have been annotated. The N50 size of 1,485 "BACtigs" consisting of redundant BACs is 337,253 bp. The largest BACtig covers 2.15 Mbp in the stickleback genome. According to the insert size distribution of mapped BACs the sea bream genome is 1.71-fold larger than the stickleback genome. These results represent a valuable tool to researchers in the field and may support future projects to elucidate the whole sea bream genome.
本研究展示了金头鲷（Sparus aurata）的首张比较BAC图谱，金头鲷是地中海地区一种极具价值的海水养殖鱼类。对一个BAC文库进行高通量末端测序产生了92,468条 reads（60.6 Mbp）。通过将BAC末端序列锚定到三刺鱼（Gasterosteus aculeatus）基因组上实现了比较图谱绘制。沿三刺鱼染色体一致排列的BAC占14,265个克隆。5,249个BAC的一部分构成了一个最小重叠路径，覆盖了三刺鱼染色体的73.5%和已注释基因的70.2%。由冗余BAC组成的1,485个“BACtigs”的N50大小为337,253 bp。最大的BACtig在三刺鱼基因组中覆盖2.15 Mbp。根据已定位BAC的插入片段大小分布，金头鲷基因组比三刺鱼基因组大1.71倍。这些结果为该领域的研究人员提供了一个有价值的工具，并可能支持未来阐明金头鲷全基因组的项目。
