Tsigenopoulos Costas S, Louro Bruno, Chatziplis Dimitrios, Lagnel Jacques, Vogiatzi Emmanouella, Loukovitis Dimitrios, Franch Rafaella, Sarropoulou Elena, Power Deborah M, Patarnello Tomaso, Mylonas Constantinos C, Magoulas Antonios, Bargelloni Luca, Canario Adelino, Kotoulas Georgios
Institute of Marine Biology, Biotechnology and Aquaculture (IMBBC), Hellenic Centre for Marine Research (HCMR), Thalassocosmos, Ex-US base at Gournes Pediados, 715 00 Heraklion, Crete, Greece.
Centro de Ciências do Mar do Algarve, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
Mar Genomics. 2014 Dec;18 Pt A:77-82. doi: 10.1016/j.margen.2014.09.008. Epub 2014 Oct 7.
An updated second linkage map was constructed for the gilthead sea bream, Sparus aurata L., a fish species of great economic importance for the Mediterranean aquaculture industry. In contrast to the first linkage map which mainly consisted of genomic microsatellites (SSRs), the new linkage map is highly enriched with SSRs found in Expressed Sequence Tags (EST-SSRs), which greatly facilitates comparative mapping with other teleosts. The new map consists of 321 genetic markers in 27 linkage groups (LGs): 232 genomic microsatellites, 85 EST-SSRs and 4 SNPs; of those, 13 markers were linked to LGs but were not ordered. Eleven markers (5 SSRs, 5 EST-SSRs and 1 SNP) are not assigned to any LG. The total length of the sex-averaged map is 1769.7cM, 42% longer than the previously published one, and the number of markers in each LG ranges from 2 to 30. The inter-marker distance varies from 0 to 75.6cM, with an average of 5.75cM. The male and female maps have a length of 1349.2 and 2172.1cM, respectively, and the average distance between markers is 4.38 and 7.05cM, respectively. Comparative mapping with the three-spined stickleback (Gasterosteus acuulatus) chromosomes and scaffolds showed conserved synteny with 132 S. aurata markers (42.9% of those mapped) having a hit on the stickleback genome.
为金头鲷（Sparus aurata L.）构建了更新的第二代连锁图谱，金头鲷是一种对地中海水产养殖业具有重要经济意义的鱼类。与主要由基因组微卫星（SSRs）组成的第一代连锁图谱不同，新的连锁图谱高度富集了在表达序列标签（EST-SSRs）中发现的微卫星，这极大地促进了与其他硬骨鱼的比较作图。新图谱由27个连锁群（LGs）中的321个遗传标记组成：232个基因组微卫星、85个EST-SSRs和4个单核苷酸多态性（SNPs）；其中，13个标记与LGs连锁但未排序。11个标记（5个SSRs、5个EST-SSRs和1个SNP）未分配到任何LGs。性别平均图谱的总长度为1769.7厘摩（cM），比之前发表的图谱长42%，每个LGs中的标记数量从2到30不等。标记间距离从0到75.6cM不等，平均为5.75cM。雄性和雌性图谱的长度分别为1349.2和2172.1cM，标记间的平均距离分别为4.38和7.05cM。与三刺鱼（Gasterosteus acuulatus）染色体和支架的比较作图显示，132个金头鲷标记（占已定位标记的42.9%）与三刺鱼基因组具有保守的同线性关系。
