Department of Prosthesis and Periodontology, Piracicaba Dental School, University of Campinas, Brazil.
J Appl Oral Sci. 2004 Sep;12(3):205-8. doi: 10.1590/s1678-77572004000300008.
Extracted human teeth are frequently used for research or educational purposes. Therefore, it is necessary to store them in disinfectant solutions that do not alter dental structures. Thus, this study evaluated the influence of storage solution on enamel demineralization. For that purpose, sixty samples were divided into the following groups: enamel stored in formaldehyde (F1), stored in thymol (T1), stored in formaldehyde and submitted to pH cycling (F2), stored in thymol and submitted to pH cycling (T2). All samples were evaluated by cross-sectional microhardness analysis and had their percentage of mineral volume versus micrometer (integrated area) determined. Differences between groups were found up to 30-µm depth from the enamel surface (p < 0.05), where samples from group T2 were more demineralized. It was concluded that the storage solution influenced the reaction of a dental substrate to a cariogenic challenge, suggesting that formaldehyde may increase enamel resistance to demineralization, when compared to demineralization occurring in enamel stored in thymol solution.
提取的人牙经常用于研究或教育目的。因此，有必要将其储存在不会改变牙结构的消毒剂溶液中。因此，本研究评估了储存溶液对牙釉质脱矿的影响。为此，将 60 个样本分为以下几组：储存在甲醛中的牙釉质（F1）、储存在百里酚中的牙釉质（T1）、储存在甲醛中并进行 pH 循环的牙釉质（F2）、储存在百里酚中并进行 pH 循环的牙釉质（T2）。所有样本均通过横截面显微硬度分析进行评估，并确定其矿物质体积与微米（积分面积）的百分比。在距牙釉质表面 30-µm 深度范围内发现了组间差异（p < 0.05），T2 组的样本脱矿程度更高。结论是储存溶液会影响牙质对致龋挑战的反应，这表明与储存在百里酚溶液中的牙釉质脱矿相比，甲醛可能会增加牙釉质的抗脱矿能力。
