Department of Medicine V, Aarhus University Hospital, Denmark.
Aliment Pharmacol Ther. 2010 Dec;32(11-12):1364-72. doi: 10.1111/j.1365-2036.2010.04463.x. Epub 2010 Sep 23.
Vitamin D3 has shown immune-modulating effects in CD4+ T cells from Crohn's disease patients in vitro.
To investigate the effects of in vivo vitamin D3 treatment on T cells in Crohn's disease patients.
Peripheral blood mononuclear cells (PBMC) were isolated at week 0 and at week 26 from 10 vitamin D3- and 10 placebo-treated Crohn's disease patients participating in a randomized, placebo-controlled, clinical trial study. Monocyte-depleted PBMC were stimulated with anti-CD3 and anti-CD28, and cultured for 7, days, to investigate CD4+ T-cell proliferation and T-cell cytokine production.
In vitamin D3-treated patients, the median 25-hydroxyvitamin D3 levels increased 70 nmol/L compared with -5 nmol/L in the placebo group. Vitamin D3 treatment increased interleukin-6 production (delta = 188 pg/mL, range: -444 to 4071) compared with a decrease in the placebo group (delta = -896 pg/mL, range: -3841 to 1323) (P < 0.02, Wilcoxon rank sum test). Interestingly, vitamin D3 increased the amount of proliferating stimulated CD4+ T cells from median 41% (range: 10-75%) to 56% (range: 26-77%) (P = 0.02, Wilcoxon rank sum test).
Vitamin D3 treatment of Crohn's disease patients increased the IL-6 levels. Interestingly, vitamin D3 treatment enhanced the CD4+ T cell proliferation.
维生素 D3 在体外已显示出对克罗恩病患者 CD4+T 细胞的免疫调节作用。
研究体内维生素 D3 治疗对克罗恩病患者 T 细胞的影响。
10 名接受维生素 D3 治疗和 10 名接受安慰剂治疗的克罗恩病患者参与了一项随机、安慰剂对照的临床试验研究，分别在第 0 周和第 26 周分离外周血单核细胞（PBMC）。用抗 CD3 和抗 CD28 刺激单核细胞耗尽的 PBMC，并培养 7 天，以研究 CD4+T 细胞增殖和 T 细胞细胞因子产生。
与安慰剂组相比，维生素 D3 治疗组患者的 25-羟维生素 D3 水平中位数增加了 70 nmol/L（-5 nmol/L）。与安慰剂组相比（-896 pg/mL，范围：-3841 至 1323），维生素 D3 治疗组白细胞介素-6 的产生增加（delta = 188 pg/mL，范围：-444 至 4071）（P < 0.02，Wilcoxon 秩和检验）。有趣的是，维生素 D3 增加了刺激的 CD4+T 细胞增殖的数量，从中位数 41%（范围：10-75%）增加到 56%（范围：26-77%）（P = 0.02，Wilcoxon 秩和检验）。
维生素 D3 治疗克罗恩病患者可增加 IL-6 水平。有趣的是，维生素 D3 治疗可增强 CD4+T 细胞的增殖。
