Department of Hematology, Xinqiao Hospital, The Third Military Medical University, Chongqing 400037, People's Republic of China.
Med Hypotheses. 2011 Feb;76(2):241-3. doi: 10.1016/j.mehy.2010.10.008. Epub 2010 Nov 2.
The bone marrow (BM) microenvironment plays an important role in regulating hematopoietic stem cell self-renewal and differentiation. Mesenchymal stem cells (MSCs), which constitute approximately 0.01-0.0001% of the nucleated cells in the adult human BM, are an important component of the BM stroma that supports hematopoiesis. The BM stroma system is often damaged in patients who have undergone high-dose chemotherapy and/or radiation treatment. Thus, the BM stroma should be reconstructed during hematopoietic stem cell transplantation (HSCT). Granulocyte-colony stimulating factor (G-CSF) is a potent hematopoietic cytokine that regulates neutrophil generation within the BM by modulating the mobility, proliferation and maturation of neutrophil progenitor cells. The results from our study here show that G-CSF markedly increased the number of donor-derived MSCs in the BM and the peripheral blood. Engraftment was faster in HSCTs with bone marrow that was treated with G-CSF (G-BM) or with G-BM- and G-CSF-treated peripheral blood stem cells compared to stead-state bone marrow (SS-BM). Based on these findings, we hypothesize that G-CSF-mobilized treatment of MSCs may accelerate engraftment in HSCT.
骨髓（BM）微环境在调节造血干细胞自我更新和分化方面起着重要作用。间充质干细胞（MSCs）构成成人 BM 有核细胞的 0.01-0.0001%左右，是支持造血的 BM 基质的重要组成部分。接受大剂量化疗和/或放射治疗的患者的 BM 基质系统经常受损。因此，在造血干细胞移植（HSCT）期间应重建 BM 基质。粒细胞集落刺激因子（G-CSF）是一种有效的造血细胞因子，通过调节中性粒细胞祖细胞的迁移、增殖和成熟来调节 BM 内中性粒细胞的生成。我们在这里的研究结果表明，G-CSF 显著增加了 BM 和外周血中供体来源的 MSC 数量。与稳态 BM（SS-BM）相比，用 G-CSF（G-BM）或 G-BM 和 G-CSF 处理的外周血干细胞处理的 BM 进行 HSCT 时，植入更快。基于这些发现，我们假设 G-CSF 动员的 MSC 治疗可能会加速 HSCT 中的植入。
