Borrelli E, Giomarelli P, Chiara O, Casini A, Betti S, Sabatini L, Lorenzini L, Grossi A
Institute of Thoracic and Cardiovascular Surgery, University of Siena, Italy.
Lung. 1990;168(1):35-42. doi: 10.1007/BF02719671.
The aim of this study was to study ultrastructural changes and lipid peroxidation in rabbits lung after massive complement activation and leukocyte aggregation. A prolonged and massive leukocyte activation was induced by intraperitoneal inoculation of zymosan suspected in paraffin. Fifteen animals (group 3) were given 0.6 g/kg of zymosan, 22 animals (group 2) received 1 g/kg, and 11 rabbits (group 1) were treated with paraffin alone and served as controls. An acute mortality rate of 40% was observed in group 3 and of 68% in group 2. Surviving animals were studied for 10 days. In these animals a marked decrease in circulating granulocytes and a progressive decline in arterial PO2 were recorded (PO2 on day 10 in group 2 animals was 51.94 +/- 4.26, p less than 0.01). Microscopic and ultrastructural evaluation revealed sequestration of granulocytes in the pulmonary microvasculature. Studies of lung homogenates demonstrated increased levels of lipid peroxide derivative malondialdehyde (group 2 rabbits, 1624 +/- 638; group 1, 795 +/- 57 pm/mg pt, p less than .001) and decreased levels of the tissue antioxidant alpha-tocopherol. The results of this study are compatible with the hypothesis of leukocyte-mediated injury through production of oxygen radicals.
本研究的目的是研究大量补体激活和白细胞聚集后兔肺的超微结构变化及脂质过氧化作用。通过腹腔接种石蜡包埋的酵母聚糖诱导白细胞长时间大量激活。15只动物（第3组）给予0.6 g/kg酵母聚糖，22只动物（第2组）给予1 g/kg，11只兔子（第1组）仅用石蜡处理作为对照。第3组的急性死亡率为40%，第2组为68%。对存活的动物进行了10天的研究。在这些动物中，记录到循环粒细胞显著减少以及动脉血氧分压逐渐下降（第2组动物第10天的血氧分压为51.94±4.26，p<0.01）。显微镜和超微结构评估显示粒细胞在肺微血管中滞留。肺匀浆研究表明脂质过氧化物衍生物丙二醛水平升高（第2组兔子，1624±638；第1组，795±57 pm/mg蛋白，p<0.001），而组织抗氧化剂α-生育酚水平降低。本研究结果与白细胞通过产生氧自由基介导损伤的假说相符。
