黑腹果蝇和奥伦纳果蝇的乙醇脱氢酶基因启动子在功能上是保守的，并且具有序列结构和核酸酶保护位点的特征。

Moses K, Heberlein U, Ashburner M

Howard Hughes Medical Institute Research Laboratories, University of California, Berkeley 94720.

Mol Cell Biol. 1990 Feb;10(2):539-48. doi: 10.1128/mcb.10.2.539-548.1990.

The sibling species Drosophila melanogaster and D. orena show similar patterns of alcohol dehydrogenase expression, both spatially and temporally. These two species diverged from a common ancestor 6 million to 15 million years ago, and the DNA sequences of the promoter regions of their Adh genes show a mosaic pattern of conservation and change. By interspecific transformation of D. orena sequences into D. melanogaster, we demonstrate a functional equivalence between these sequences. Using both D. melanogaster embryo extracts and purified transcription factor Adf-1, we compare the protection of these promoter sequences from nuclease, demonstrating considerable conservation.

同胞物种黑腹果蝇和奥伦纳果蝇在酒精脱氢酶表达模式上，无论在空间还是时间上都表现出相似性。这两个物种在600万至1500万年前从一个共同祖先分化而来，它们Adh基因启动子区域的DNA序列呈现出保守与变化的镶嵌模式。通过将奥伦纳果蝇的序列进行种间转化到黑腹果蝇中，我们证明了这些序列之间的功能等效性。利用黑腹果蝇胚胎提取物和纯化的转录因子Adf-1，我们比较了这些启动子序列对核酸酶的抗性，结果表明存在相当程度的保守性。