Department of Morphology, Stomatology and Physiology, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil.
Am J Orthod Dentofacial Orthop. 2010 Nov;138(5):613-6. doi: 10.1016/j.ajodo.2010.01.029.
Orthodontic tooth movement uses mechanical forces that result in inflammation in the first days. Myeloperoxidase (MPO) is an enzyme found in polymorphonuclear neutrophil (PMN) granules, and it is used to estimate the number of PMN granules in tissues. So far, MPO has not been used to study the inflammatory alterations after the application of orthodontic tooth movement forces. The aim of this study was to determine MPO activity in the gingival crevicular fluid (GCF) and saliva (whole stimulated saliva) of orthodontic patients at different time points after fixed appliance activation.
MPO was determined in the GCF and collected by means of periopaper from the saliva of 14 patients with orthodontic fixed appliances. GCF and saliva samples were collected at baseline, 2 hours, and 7 and 14 days after application of the orthodontic force.
Mean MPO activity was increased in both the GCF and saliva of orthodontic patients at 2 hours after appliance activation (P <0.02 for all comparisons). At 2 hours, PMN infiltration into the periodontal ligament from the orthodontic force probably results in the increased MPO level observed at this time point.
MPO might be a good marker to assess inflammation in orthodontic movement; it deserves further studies in orthodontic therapy.
正畸牙齿移动利用机械力，在最初几天会导致炎症。髓过氧化物酶（MPO）是一种存在于多形核中性粒细胞（PMN）颗粒中的酶，用于估计组织中PMN 颗粒的数量。到目前为止，MPO 尚未用于研究正畸牙齿移动力应用后的炎症变化。本研究旨在确定正畸固定矫治器激活后不同时间点患者龈沟液（GCF）和唾液（全刺激唾液）中的 MPO 活性。
使用牙周纸从 14 名正畸固定矫治器患者的唾液中收集 GCF，并用 MPO 测定。在应用正畸力之前、之后 2 小时以及 7 天和 14 天收集 GCF 和唾液样本。
正畸患者在矫治器激活后 2 小时，GCF 和唾液中的平均 MPO 活性均增加（所有比较均 P <0.02）。在 2 小时时，正畸力可能导致牙周韧带中 PMN 的浸润，从而导致此时观察到的 MPO 水平升高。
MPO 可能是评估正畸运动中炎症的一个很好的标志物；它值得在正畸治疗中进一步研究。
