Perinetti Giuseppe, Serra Emanuela, Paolantonio Michele, Bruè Claudia, Meo Silvio Di, Filippi Maria Rita, Festa Felice, Spoto Giuseppe
Department of Oral Sciences, School of Dentistry, University of G. D'Annunzio, Chieti, Italy.
J Periodontol. 2005 Mar;76(3):411-7. doi: 10.1902/jop.2005.76.3.411.
During orthodontic tooth movement, the early response of periodontal tissues to mechanical stress is an acute inflammatory one. This study uses a longitudinal design to examine lactate dehydrogenase (LDH) activity in gingival crevicular fluid (GCF) to determine if GCF LDH can be used as a diagnostic aid in monitoring tooth movement and tissue response during orthodontic treatment.
Seventeen patients (mean age: 16.1 years) participated in the study. Each patient was undergoing treatment for distal movement, and an upper first molar served as the test tooth (TT), while the contralateral (CT) and antagonist (AT) teeth were used as controls. The CT was included in the orthodontic appliance, but was not subjected to the distal movement; the AT was free from any orthodontic appliance. The GCF around the experimental teeth was harvested from both mesial and distal tooth sites immediately before appliance activation, and on days 7, 14, and 21. Clinical gingival conditions were also recorded.
Gingival crevicular fluid LDH activity was significantly elevated in all sites of the TT and CT, as compared to the AT, where LDH activity remained at the baseline level throughout the study. Enzyme activity levels were also greater in the TT than in the CT, and in the compression sites.
Our results suggest that GCF LDH levels reflect the biological activity that takes place in the periodontium during orthodontic movement, and therefore they can be used as a diagnostic tool for monitoring for correct orthodontic tooth movement in clinical practice.
在正畸牙齿移动过程中，牙周组织对机械应力的早期反应是急性炎症反应。本研究采用纵向设计，检测龈沟液（GCF）中乳酸脱氢酶（LDH）活性，以确定GCF中的LDH是否可作为正畸治疗期间监测牙齿移动和组织反应的诊断辅助指标。
17名患者（平均年龄：16.1岁）参与了本研究。每位患者均在进行远中移动治疗，将上颌第一磨牙作为受试牙（TT），对侧牙（CT）和拮抗牙（AT）作为对照。CT纳入正畸矫治器，但不进行远中移动；AT未佩戴任何正畸矫治器。在矫治器激活前以及第7、14和21天，从受试牙的近中及远中牙位采集实验牙周围的GCF。同时记录临床牙龈状况。
与AT相比，TT和CT所有部位的龈沟液LDH活性均显著升高，而AT在整个研究过程中LDH活性保持在基线水平。酶活性水平在TT中也高于CT，且在受压部位更高。
我们的结果表明，GCF中的LDH水平反映了正畸移动过程中牙周组织发生的生物学活性，因此在临床实践中可作为监测正畸牙齿正确移动的诊断工具。
