Navarro-Palacios Alejandra, García-López Eliezer, Meza-Rios Alejandra, Armendariz-Borunda Juan, Sandoval-Rodríguez Ana
Postgraduate student, Department of Orthodontics, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Professor and chairman, Department of Orthodontics, University of Guadalajara, Guadalajara, Jalisco, Mexico.
Am J Orthod Dentofacial Orthop. 2014 Jul;146(1):92-7. doi: 10.1016/j.ajodo.2014.04.015.
Orthodontic tooth movement implies application of forces that generate an inflammatory process. The myeloperoxidase (MPO) enzyme is found inside neutrophil granules. MPO activity indirectly reflects the level of inflammation. The aim of this study was to measure MPO activity in gingival crevicular fluid (GCF) and whole saliva in orthodontic patients with different levels of dental crowding at the alignment phase of orthodontic treatment with the same archwires.
Twenty patients were classified according to the irregularity index into 2 groups: severe and minimum crowding (10 in each group). MPO activity was evaluated in GCF and saliva at 0 and 2 hours, and 7 and 14 days after the orthodontic appliances were activated. MPO activity was measured using the modified Bradley-Bozeman technique.
In both groups, the maximum activity was at 2 hours (P <0.05) after activation. MPO activity remained elevated until day 7, and values similar to baseline were found at day 14 in the GCF and saliva samples. Enzymatic activity did not show statistical differences between the groups.
The amount of dental crowding does not seem to influence MPO activity, which showed similar patterns in GCF and saliva, but the values in GCF reflected the inflammatory changes more accurately than did the values in saliva. The quantification of MPO activity is a useful biologic marker as an indirect measurement of inflammation generated with tooth movement independent of the amount of crowding.
正畸牙齿移动意味着施加能引发炎症过程的力。髓过氧化物酶（MPO）存在于中性粒细胞颗粒内。MPO活性间接反映炎症水平。本研究的目的是在使用相同弓丝进行正畸治疗排齐阶段的不同牙列拥挤程度的正畸患者中，测量龈沟液（GCF）和全唾液中的MPO活性。
根据不齐指数将20名患者分为2组：严重拥挤组和最小拥挤组（每组10名）。在正畸矫治器激活后0小时、2小时、7天和14天评估GCF和唾液中的MPO活性。使用改良的Bradley - Bozeman技术测量MPO活性。
两组在激活后2小时均出现最大活性（P <0.05）。MPO活性在第7天前一直升高，在第14天GCF和唾液样本中发现其值与基线相似。两组间酶活性无统计学差异。
牙列拥挤程度似乎不影响MPO活性，其在GCF和唾液中表现出相似模式，但GCF中的值比唾液中的值更准确地反映炎症变化。MPO活性的定量是一种有用的生物学标志物，可作为独立于拥挤程度的牙齿移动所产生炎症的间接测量指标。
