一种新型集落印迹免疫测定法揭示了四种不相关的外生菌根真菌病毒在Rosellinia necatrix 中的分布和水平传播的差异模式。
A novel colony-print immunoassay reveals differential patterns of distribution and horizontal transmission of four unrelated mycoviruses in Rosellinia necatrix.
机构信息
Apple Research Station, National Institute of Fruit Tree Science, National Agricultural and Food Research Organization (NARO), Morioka 020-0123, Japan.
出版信息
Virology. 2011 Jan 20;409(2):280-9. doi: 10.1016/j.virol.2010.10.014. Epub 2010 Nov 5.
A colony-print immunoassay (CPIA) using an anti-dsRNA antibody was developed to visualize the distribution of four unrelated mycoviruses with dsRNA genomes, a partitivirus (RnPV1), mycoreovirus (RnMyRV3), megabirnavirus (RnMBV1), and an unidentified virus (RnQV1), in mycelia of the white root rot fungus, Rosellinia necatrix. CPIA revealed different distribution patterns within single colonies for each virus. Both RnPV1 and RnMBV1 were distributed throughout single colonies, RnMyRV3 was absent from some colony sectors, and RnQV1 exhibited varied accumulation levels between sectors. RnMyRV3 and RnQV1 were transmitted to the recipient virus-free colonies of virus-infected and virus-free colony pairs more slowly than were RnPV1 or RnMBV1. The presence of RnMyRV3 in recipient colonies restricted horizontal transmission of RnPV1 and RnMBV1. These results imply that one or more mechanisms are present in host-virus and virus-virus interactions that restrict the spread of viruses within and between colonies.
采用抗 dsRNA 抗体的集落印迹免疫分析（CPIA）被开发用于可视化具有 dsRNA 基因组的四种不相关真菌病毒的分布，包括部分病毒（RnPV1）、真菌虹彩病毒（RnMyRV3）、巨型双 RNA 病毒（RnMBV1）和一种未鉴定的病毒（RnQV1），这些病毒存在于白根腐真菌 Rosellinia necatrix 的菌丝体中。CPIA 揭示了每种病毒在单个菌落内的不同分布模式。RnPV1 和 RnMBV1 均分布于单个菌落中，RnMyRV3 不存在于某些菌落区，而 RnQV1 在区之间表现出不同的积累水平。RnMyRV3 和 RnQV1 向受感染和无病毒菌落对的受体无病毒菌落中的传播速度慢于 RnPV1 或 RnMBV1。受感染菌落中 RnMyRV3 的存在限制了 RnPV1 和 RnMBV1 的水平传播。这些结果表明，在宿主-病毒和病毒-病毒相互作用中存在一种或多种机制，限制了病毒在菌落内部和之间的传播。
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