通过完整的体内感染周期，研究 GFP 转化的边缘无浆体的稳定性和 tick 传播表型。
Stability and tick transmission phenotype of gfp-transformed Anaplasma marginale through a complete in vivo infection cycle.
机构信息
Animal Disease Research Unit, Agriculture Research Service, US Department of Agriculture, Pullman, WA 99164-7030, USA.
出版信息
Appl Environ Microbiol. 2011 Jan;77(1):330-4. doi: 10.1128/AEM.02096-10. Epub 2010 Nov 5.
Abstract
We tested the stability and tick transmission phenotype of transformed Anaplasma marginale through a complete in vivo infection cycle. Similar to the wild type, the gfp-transformed A. marginale strain established infection in cattle, a natural reservoir host, and persisted in immune competent animals. The tick infection rates for the transformed A. marginale and the wild type were the same. However, there were significantly lower levels of the transformed A. marginale than of the wild type in the tick. Despite the lower levels of replication, ticks transmitted the transformant. Transformants can serve as valuable tools to dissect the molecular requirements of tick colonization and pathogen transmission.
摘要
我们通过完整的体内感染周期来测试转化的边缘无浆体的稳定性和蜱传表型。与野生型相似，gfp 转化的边缘无浆体菌株在牛（一种天然的储存宿主）中建立了感染，并在免疫功能正常的动物中持续存在。转化的边缘无浆体和野生型的蜱感染率相同。然而，蜱中的转化的边缘无浆体水平明显低于野生型。尽管复制水平较低，但蜱仍能传播转化体。转化体可以作为一种有价值的工具，用于剖析蜱定殖和病原体传播的分子要求。
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