Rebl Henrike, Finke Birgit, Schroeder Karsten, Nebe J Barbara
University of Rostock, Biomedical Research Center, Dept. of Cell Biology, Rostock, Germany.
Int J Artif Organs. 2010 Oct;33(10):738-48.
To improve orthopedic implant ingrowth, knowledge of the effect of chemical surface modifications on vital cell function in vitro is of importance. Early in our investigations we recognized that amino groups, positively charged via plasma polymerized allylamine, increased cell growth and the actin-filament formation in the initial cell-material contact phase. To gain insight into continuous vital cell behavior on this plasma polymer layer, here we present the metabolic activity of osteoblasts and their time-dependent adhesion using the sensor chip technology.
We demonstrate a new method for continuous 24 hour-measurements with vital human osteoblast-like cells (MG-63, ATCC) on sensor chips (Bionas® SC 1000) modified with plasma polymerized allylamine (PPAAm). The PPAAm film deposited on the chip is a cross-linked, strongly fixed plasma polymer with relatively high amino functionality and well defined chemical surface composition. We assessed continuous cell adhesion and the metabolic activity, i.e., oxygen consumption and acidification.
We determined that adhesion of vital cells on PPAAm is not only enhanced shortly (1 h) after cell seeding but remained continuously higher for 24 h, which is significant. This nanometer-thin PPAAm layer did not change the overall metabolic activity of MG-63 cells during 24 h.
This tool--using adhesion and metabolic sensor chips--appears to be a suitable method for the recognition of vital cell physiology in biocompatibility measurements of plasma chemical treated surfaces.
为了改善骨科植入物的骨长入情况，了解化学表面修饰对体外活细胞功能的影响至关重要。在我们早期的研究中，我们认识到通过等离子体聚合烯丙胺带正电荷的氨基在初始细胞 - 材料接触阶段会增加细胞生长和肌动蛋白丝的形成。为了深入了解活细胞在该等离子体聚合物层上的持续行为，在此我们使用传感器芯片技术展示成骨细胞的代谢活性及其时间依赖性粘附。
我们展示了一种新方法，用于在经等离子体聚合烯丙胺（PPAAm）修饰的传感器芯片（Bionas® SC 1000）上对人成骨样活细胞（MG - 63，ATCC）进行连续24小时测量。沉积在芯片上的PPAAm膜是一种交联的、牢固固定的等离子体聚合物，具有相对较高的氨基官能度和明确的化学表面组成。我们评估了细胞的持续粘附以及代谢活性，即氧气消耗和酸化情况。
我们确定活细胞在PPAAm上的粘附不仅在细胞接种后不久（1小时）增强，而且在24小时内持续保持较高水平，这具有重要意义。这种纳米级薄的PPAAm层在24小时内并未改变MG - 63细胞的整体代谢活性。
这种使用粘附和代谢传感器芯片的工具似乎是一种在等离子体化学处理表面的生物相容性测量中识别活细胞生理学的合适方法。
