Rebl Henrike, Finke Birgit, Schmidt Jürgen, Mohamad Heba S, Ihrke Roland, Helm Christiane A, Nebe J Barbara
Dept. of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, Germany.
Leibniz-Institute for Plasma Science and Technology e.V. (INP), Felix-Hausdorff-Str. 2, 17489 Greifswald, Germany.
Mater Sci Eng C Mater Biol Appl. 2016 Dec 1;69:1116-24. doi: 10.1016/j.msec.2016.08.016. Epub 2016 Aug 9.
Excellent osseointegration of permanent implants is crucial for the long lasting success of the implantation. To improve the osseointegrative potential, bio-inert titanium alloy surfaces (Ti6Al4V) are modified by plasma chemical oxidation (PCO®) of the titanium-oxide layer to a non-stoichiometric, amorphous calcium phosphate layer. The native titanium-oxide film measuring only a few nanometers is converted by PCO® to a thick porous calcium phosphate layer of about 10μm. In a second step the PCO surface is combined with a cell adhesive plasma-polymerized allylamine (PPAAm) nano film (5 and 50nm). Independent of the PPAAm coating homogeneity, the human osteoblast-like MG-63 cells show a remarkable increase in cell size and well-developed filopodia. Analyses of the actin cytoskeleton reveal that the cells mold to the pore shape of the PPAAm-covered PCO, thereby establishing a strong attachment to the surface. Interestingly, we could demonstrate that even though our untreated PCO shows excellent hydrophilicity, this alone is not sufficient to facilitate fast cell spreading, but the positive surface charges mediated by PPAAm. This multilayer composite material guarantees enhanced interlocking of the cells with the porous surface.
永久植入物的良好骨整合对于植入的长期成功至关重要。为了提高骨整合潜力，通过对生物惰性钛合金表面（Ti6Al4V）的氧化钛层进行等离子体化学氧化（PCO®），将其改性为非化学计量的无定形磷酸钙层。仅几纳米厚的天然氧化钛膜通过PCO®转化为约10μm厚的厚多孔磷酸钙层。在第二步中，将PCO表面与细胞粘附性等离子体聚合烯丙胺（PPAAm）纳米膜（5和50nm）结合。无论PPAAm涂层的均匀性如何，人成骨样MG-63细胞的细胞大小都显著增加，丝状伪足发育良好。对肌动蛋白细胞骨架的分析表明，细胞会根据PPAAm覆盖的PCO的孔形状进行塑形，从而在表面上建立牢固的附着。有趣的是，我们可以证明，即使我们未处理的PCO具有优异的亲水性，但仅靠这一点不足以促进细胞快速铺展，而是PPAAm介导的正表面电荷起到了作用。这种多层复合材料保证了细胞与多孔表面之间增强的互锁。
