Namsaraev Z B, Babasanova O B, Dunaevsky Y E, Akimov V N, Barkhutova D D, Gorlenko V M, Namsaraev B B
Winogradsky Institute of Microbiology RAS, pr. 60-letiya Oktyabrya 7/2, Moscow, 117312, Russia.
Mikrobiologiia. 2010 Jul-Aug;79(4):516-23.
A Gram reaction positive, spore-forming, facultative anaerobic bacterium belonging to the Phylum Firmicutes, was isolated from alkaline hot (80 degrees C, pH 9.8 spring Tsenher, central Mongolia. The cells were rod shaped, feebly motile, peritrichously flagellated. Strain T4 was moderately thermophilic with optimum growth at 60 degrees C. Maximum temperature for growth was between 70 and 75 degrees C; minimum temperature for growth was between 35 and 30 degrees C. Alkalitolerant, optimum pH for growth was 8.0; minimum pH for growth was between 5.0 and 5.5 and maximum was between 10.5 and 10.8. The growth was observed at NaCl concentrations of 0-5% (w/v) with the optimum at 0.2-0.5%. No growth was observed at 6% NaCl (w/v). Aerobically, the strain utilized proteinaceous substrates, organic acids and a range of carbohydrates including glucose, ribose, sucrose and xylose as well. Anaerobically, only glucose and sucrose were utilized. Strain T4T produced thermostable alkaline subtilisin-like serine proteinase. The G + C content was 44.2 mol. % (td). On the basis of 16S rRNA gene sequence similarity strain T4(T) was shown to be closely related to the members of the genus Anoxybacillus (family Bacillaceae, class "Bacilli"). DNA-DNA hybridization data revealed that strain T4T had only 38% relatedness to A. flavithermus and 28% relatedness to A. pushchinoensis. Based on its morphology, physiology, phylogenetic relationship and its low DNA-DNA relatedness values with validly published species of Anoxybacillus, it is proposed that strain T4T represents a novel species Anoxybacillus mongoliensis sp. nov., with the type strain T4(T) (=DSM 19169 = VKM 2407).
从蒙古国中部的察干海尔碱性热泉（80℃，pH 9.8）中分离出一株革兰氏阳性、形成芽孢、兼性厌氧的厚壁菌门细菌。细胞呈杆状，运动能力较弱，周生鞭毛。菌株T4为中度嗜热菌，最适生长温度为60℃。生长的最高温度在70至75℃之间；生长的最低温度在35至30℃之间。耐碱，最适生长pH为8.0；生长的最低pH在5.0至5.5之间，最高在10.5至10.8之间。在NaCl浓度为0 - 5%（w/v）时观察到生长，最适浓度为0.2 - 0.5%。在6% NaCl（w/v）时未观察到生长。有氧条件下，该菌株利用含蛋白质底物、有机酸以及一系列碳水化合物，包括葡萄糖、核糖、蔗糖和木糖。厌氧条件下，仅利用葡萄糖和蔗糖。菌株T4T产生耐热碱性枯草杆菌蛋白酶样丝氨酸蛋白酶。G + C含量为44.2 mol.%（td）。基于16S rRNA基因序列相似性，菌株T4(T)被证明与嗜热栖热放线菌属（芽孢杆菌科，芽孢杆菌纲）的成员密切相关。DNA - DNA杂交数据显示，菌株T4T与嗜热栖热放线菌的相关性仅为38%，与普希诺嗜热放线菌的相关性为28%。基于其形态、生理、系统发育关系以及与已有效发表的嗜热栖热放线菌属物种的低DNA - DNA相关性值，建议将菌株T4T代表一个新物种——蒙古嗜热栖热放线菌新种，模式菌株为T4(T)（=DSM 19169 = VKM 2407）。
