Department of Orthopaedics, School of Medicine, West Virginia University, Morgantown, West Virginia 26506, United States.
Biomacromolecules. 2010 Dec 13;11(12):3630-7. doi: 10.1021/bm1010855. Epub 2010 Nov 8.
The current state-of-the-art for drug-carrying biomedical devices is mostly limited to those that release a single drug. Yet there are many situations in which more than one therapeutic agent is needed. Also, most polyelectrolyte multilayer films intended for drug delivery are loaded with active molecules only during multilayer film preparation. In this paper, we present the integration of capsules as vehicles within polypeptide multilayer films for sustained release of multiple oppositely charged drug molecules using layer-by-layer nanoassembly technology. Calcium carbonate (CaCO(3)) particles were impregnated with polyelectrolytes, shelled with polyelectrolyte multilayers, and then assembled onto polypeptide multilayer films using glutaraldehyde. Capsule-integrated polypeptide multilayer films were obtained after decomposition of CaCO(3) templates. Two oppositely charged drugs were loaded into capsules within polypeptide multilayer films postpreparation based on electrostatic interactions between the drugs and the polyelectrolytes impregnated within capsules. We determined that the developed innovative capsule-integrated polypeptide multilayer films could be used to load multiple drugs of very different properties (e.g., opposite charges) any time postpreparation (e.g., minutes before surgical implantation inside an operating room), and such capsule-integrated films allowed simultaneous delivery of two oppositely charged drug molecules and a sustained (up to two weeks or longer) and sequential release was achieved.
载药生物医学器件的最新技术大多局限于只能释放一种药物的器件。然而，在许多情况下，需要使用多种治疗剂。此外，大多数用于药物输送的聚电解质多层膜仅在多层膜制备过程中装载活性分子。在本文中，我们提出了将胶囊作为载体整合到多肽多层膜中，以使用层层纳米组装技术持续释放多种带相反电荷的药物分子。碳酸钙 (CaCO(3)) 颗粒用聚电解质浸渍，用聚电解质多层包裹，然后用戊二醛组装到多肽多层膜上。在 CaCO(3) 模板分解后，获得了胶囊整合的多肽多层膜。两种带相反电荷的药物基于药物与胶囊内浸渍的聚电解质之间的静电相互作用，在多肽多层膜制备后被加载到胶囊内。我们确定，开发的创新胶囊整合多肽多层膜可以在制备后（例如在手术室中进行手术植入前几分钟）随时加载多种性质非常不同的药物（例如，相反电荷），并且这种胶囊整合的薄膜允许同时输送两种带相反电荷的药物分子，并实现持续（长达两周或更长时间）和顺序释放。
