Limpeanchob Nanteetip, Trisat Kanittaporn, Duangjai Acharaporn, Tiyaboonchai Waree, Pongcharoen Sutatip, Sutheerawattananonda Manote
Department of Pharmacy Practice and Center of Excellence for Innovation in Chemistry.
J Agric Food Chem. 2010 Dec 8;58(23):12519-22. doi: 10.1021/jf103157w. Epub 2010 Nov 8.
A cholesterol lowering effect of sericin was investigated both in vivo and in vitro. Rats were dosed with cholesterol with and without sericin for 14 days. Non-high-density lipoprotein (HDL) and total serum cholesterols were reduced in rats fed high-cholesterol diet with all three tested doses of sericin (10, 100, and 1000 mg kg(-1) day(-1)). The potential mechanism of actions was determined by measuring the uptake of radiolabeled cholesterol into differentiated Caco-2 cells and cholesterol solubility in mixed lipid micelles. Concentration of sericin as low as 25 and 50 μg/mL inhibited 30% of cholesterol uptake into Caco-2 cells whereas no effect was found at higher concentration. Cholesterol micellar solubility was reduced in the presence of sericin. This study suggests the cholesterol lowering effect of sericin results from its inhibition of cholesterol absorption in intestinal cells and its reduction of cholesterol solubility in lipid micelles.
在体内和体外研究了丝胶蛋白的降胆固醇作用。给大鼠喂食含胆固醇的食物，同时分别添加和不添加丝胶蛋白，持续14天。在喂食高胆固醇饮食并添加三种测试剂量丝胶蛋白（10、100和1000 mg kg(-1) 天(-1)）的大鼠中，非高密度脂蛋白（HDL）和总血清胆固醇均有所降低。通过测量放射性标记胆固醇进入分化的Caco-2细胞的摄取量以及胆固醇在混合脂质微团中的溶解度来确定其潜在作用机制。低至25和50 μg/mL的丝胶蛋白浓度可抑制30%的胆固醇进入Caco-2细胞，而在较高浓度下未发现影响。在丝胶蛋白存在的情况下，胆固醇微团溶解度降低。这项研究表明，丝胶蛋白的降胆固醇作用源于其对肠道细胞中胆固醇吸收的抑制以及对脂质微团中胆固醇溶解度的降低。
