Hindmarch J M, Magee J T, Hadfield M A, Duerden B I
Department of Bacteriology, Royal Hallamshire Hospital, Sheffield.
J Med Microbiol. 1990 Feb;31(2):137-49. doi: 10.1099/00222615-31-2-137.
Clinical (66) and collection (38) strains of Corynebacterium spp., including C. jeikeium and CDC group D2, and of Listeria monocytogenes were examined. Conventional characters used in species identification were assessed by a microbiochemical method, and pyrolysis-mass spectrometry (Py-MS) was performed with a Horizon Instruments PYMS 200X. Classification based on Py-MS data yielded clusters that corresponded with species identification and classification groups from conventional data. One small group of clinical strains, homogeneous in conventional tests and Py-MS, comprised isolates from sputum samples from patients undergoing ventilation; they were similar to collection strains of C. renale and C. striatum; the latter species has been implicated in chest infection. Another group, similar to C. minutissimum in both systems, comprised clinical strains isolated from urogenital specimens. L. monocytogenes strains were clearly distinct from Corynebacterium spp. Groups comprising CDC D2 strains and C. jeikeium were resolved, and were similar to other Corynebacterium spp. Two collection strains of C. xerosis were distinct in conventional tests and Py-MS.
对包括杰氏棒杆菌和疾病预防控制中心D2组在内的棒状杆菌属以及单核细胞增生李斯特菌的66株临床菌株和38株收集菌株进行了检测。通过微生物化学方法评估用于菌种鉴定的常规特征，并使用Horizon Instruments PYMS 200X进行热解质谱分析（Py-MS）。基于Py-MS数据的分类产生了与常规数据的菌种鉴定和分类组相对应的聚类。一组在常规测试和Py-MS中表现均一的临床菌株，由来自接受通气治疗患者痰液样本的分离株组成；它们与肾棒杆菌和纹带棒杆菌的收集菌株相似；后者与胸部感染有关。在两个系统中均与极小棒杆菌相似的另一组，由从泌尿生殖系统标本中分离出的临床菌株组成。单核细胞增生李斯特菌菌株与棒状杆菌属明显不同。包含疾病预防控制中心D2组菌株和杰氏棒杆菌的组得以区分，且与其他棒状杆菌属相似。两株干燥棒杆菌的收集菌株在常规测试和Py-MS中表现不同。
