Magee J T, Hindmarch J M, Burnett I A, Pease A
Department of Microbiology, Children's Hospital, Sheffield.
J Med Microbiol. 1989 Dec;30(4):273-8. doi: 10.1099/00222615-30-4-273.
Strains of Streptococcus pyogenes from an outbreak of infection on a burns unit (15), a collection of routine isolates from another hospital(12) and isolates from a national survey of throat infections in children in the community(4) were examined blind by pyrolysis-mass spectrometry (Py-MS). The outbreak strains (M22 T12) previously found to give identical typing results in conventional tests, formed a closely similar cluster and were distinct from other hospital and community strains. One hospital and one community strain were loosely associated with this cluster. Another cluster comprised six antibiotic-susceptible strains and two community strains. Six strains did not fall within the clusters; four were antibiotic-resistant strains isolated in hospital, one an antibiotic-resistant strain isolated in the community, and one a susceptible hospital strain. Results show that Py-MS is a potentially valuable method for rapid comparison of strains in studies of infection.
对来自一家烧伤病房感染暴发的化脓性链球菌菌株（15株）、另一家医院的常规分离菌株（12株）以及社区儿童全国性咽喉感染调查中的分离菌株（4株），采用热解质谱法（Py-MS）进行了盲法检测。此前发现在传统检测中给出相同分型结果的暴发菌株（M22 T12），形成了一个非常相似的簇，且与其他医院和社区菌株不同。一株医院菌株和一株社区菌株与该簇有松散关联。另一个簇包含6株抗生素敏感菌株和2株社区菌株。6株菌株不属于这些簇；4株是在医院分离的耐药菌株，1株是在社区分离的耐药菌株，1株是医院的敏感菌株。结果表明，在感染研究中，热解质谱法是一种用于快速比较菌株的潜在有价值的方法。
