Freeman R, Goodfellow M, Gould F K, Hudson S J, Lightfoot N F
Department of Microbiology, University of Newcastle upon Tyne.
J Med Microbiol. 1990 Aug;32(4):283-6. doi: 10.1099/00222615-32-4-283.
Fresh clinical isolates of Salmonella spp. and Streptococcus pyogenes were analysed by pyrolysis-mass spectrometry (Py-MS). The results formed the basis of mathematically derived characterizations of individual strains and these were compared with the results of phage typing for the salmonellas and M protein typing for the streptococci. Py-MS was shown to be a rapid and reproducible method for inter-strain comparisons, giving evidence of identity and non-identity between strains that agreed well with the results of conventional tests. Py-MS has potential value as a rapid, relatively inexpensive and highly discriminatory method of epidemiological analysis in bacterial disease.
采用热解质谱法（Py-MS）对沙门氏菌属和化脓性链球菌的新鲜临床分离株进行了分析。这些结果构成了对各个菌株进行数学推导特征描述的基础，并将其与沙门氏菌的噬菌体分型结果以及链球菌的M蛋白分型结果进行了比较。结果表明，Py-MS是一种用于菌株间比较的快速且可重复的方法，能够提供菌株间相同与不同的证据，与传统检测结果高度吻合。Py-MS作为一种快速、相对廉价且具有高度鉴别力的细菌病流行病学分析方法具有潜在价值。
