Northeast Forestry University, Harbin, China.
Am J Chin Med. 2010;38(6):1107-14. doi: 10.1142/S0192415X10008500.
Water decoctions from the leaves of Taxus cuspidata are used in traditional Chinese medicine to treat cancer, suggesting that water soluble constituents from these leaves may possess anticancer properties. Interestingly, hydrophilic paclitaxel derivatives, as opposed to paclitaxel itself, can be detected by high pressure liquid chromatography in water decoctions from these leaves. The remainder extracts, which are free of paclitaxel and hydrophilic paclitaxel derivatives, from the T. cuspidata leaves were investigated for antitumor activity in vivo and in vitro for the first time in this study. EE80B, 7-xylosyl-10-deacetylpaclitaxel and 7-xylosyl-10-deacetylpaclitaxel C displayed the most antitumor activity in vivo. However, in vitro studies with tumor cell lines showed that EE80B had a significantly smaller antitumor effect than paclitaxel. We hypothesize that water decoctions from T. cuspidata leaves exhibit antitumor effects in vivo, which may be aided by the activation of specific host mechanisms (e.g. stimulation of antitumor immunity) which are not present in vitro.
从红豆杉的叶子中提取的水煎剂被用于中医治疗癌症，这表明这些叶子中的水溶性成分可能具有抗癌特性。有趣的是，与紫杉醇本身不同，亲水紫杉醇衍生物可以通过高效液相色谱法在这些叶子的水煎剂中检测到。在这项研究中，首次研究了红豆杉叶子中剩余的、不含紫杉醇和亲水紫杉醇衍生物的提取物的体内和体外抗肿瘤活性。EE80B、7-木糖基-10-去乙酰紫杉醇和 7-木糖基-10-去乙酰紫杉醇 C 在体内显示出最强的抗肿瘤活性。然而，肿瘤细胞系的体外研究表明，EE80B 的抗肿瘤作用明显小于紫杉醇。我们假设红豆杉叶子的水煎剂在体内具有抗肿瘤作用，这可能是由于体内存在特定的宿主机制（例如刺激抗肿瘤免疫）的激活，而在体外则不存在。
