Departments of Chemistry and Materials Science and Engineering, University of Washington, Seattle, Washington 98195-2120, USA.
ACS Appl Mater Interfaces. 2010 Nov;2(11):3153-9. doi: 10.1021/am100635v. Epub 2010 Nov 9.
The fluorescence from conjugated polymer assembled onto lithographically fabricated gold nanoarrays using genetically engineered peptides as molecular linkers is studied. A 16-fold increase in the photoluminescence of the conjugated polymer is observed when assembled on the optimized nanostructures due to surface plasmon enhanced fluorescence. This is achieved using a water-soluble cationic conjugated polymer, poly[(9,9-bis(6'-((N,N,N-trimethylammonium)hexyl)-2,7-fluorene)-co-4,7-di-2-thienyl-2,1,3-benzothiadiazole] dibromide (PFDBT-N(+)), systematically tuning the vertical distance of PFDBT-N(+) from the gold nanopillar surface using solid-specific peptide linkers and horizontally optimizing the localized surface plasmon resonance by varying the geometric arrangements of the patterned metal nanoarrays. The diameter and tip-to-tip spacing of the nanopillars along with vertically tuning the distance of PFDBT-N(+) from the nanopillar affected the observed fluorescence enhancements. The collective optical properties of conjugated polymers combined with the photonic properties of nanoparticles provide a new means in the development of metal enhanced hybrid nanomaterials for biotechnology.
研究了通过遗传工程肽作为分子接头将共轭聚合物组装到光刻制作的金纳米阵列上的荧光。由于表面等离子体增强荧光，当在优化的纳米结构上组装时，共轭聚合物的光致发光强度增加了 16 倍。这是通过使用水溶性阳离子共轭聚合物聚[(9,9-双(6'-((N,N,N-三甲基铵)己基)-2,7-芴)-共-4,7-二-2-噻吩基-2,1,3-苯并噻二唑]二溴化物(PFDBT-N(+))来实现的，通过使用固态特异性肽接头系统地调节 PFDBT-N(+)从金纳米柱表面的垂直距离，并通过改变图案化金属纳米阵列的几何排列来水平优化局域表面等离子体共振。纳米柱的直径和尖端到尖端间距以及垂直调节 PFDBT-N(+)从纳米柱的距离影响了观察到的荧光增强。共轭聚合物的集体光学性质与纳米颗粒的光子性质相结合，为生物技术中金属增强杂化纳米材料的发展提供了一种新的手段。
