Japan Society for the Promotion of Science.
Phytopathology. 2010 Dec;100(12):1289-97. doi: 10.1094/PHYTO-04-10-0109.
Pine wilt disease, caused by the pine wood nematode (PWN), Bursaphelenchus xylophilus, has become of worldwide quarantine concern in recent years. Here, we disclosed the surface coat (SC) proteins of the PWN which are thought to be one of the key components in pine wilt development. This is the first report that focused on the SC proteins and thoroughly identified those proteins of a plant-parasitic nematode using the proteomic approach. In this study, SC protein profiles were compared for PWNs grown on the fungus Botrytis cinerea and in host pine seedlings. The results demonstrated that the gross amount of PWN SC proteins drastically increased during infection of the host pine. Thirty-seven protein bands showed significant quantity differences between fungus-grown and host-origin PWNs, and were used for identification by matrix-assisted laser desorption ionization time of flight mass spectrometry analysis. These included several proteins that are presumed to be involved in the host immune response; for example, regulators of reactive oxygen species (ROS) and a ROS scavenger. These results might suggest that the PWN SC proteins are crucial in modulating or evading host immune response. Our data provide a new insight into the mechanism of pine wilt disease and the biological role of the SC proteins of plant-parasitic nematodes.
松材线虫病由松材线虫（Bursaphelenchus xylophilus）引起，近年来已成为全球检疫关注的问题。在这里，我们揭示了松材线虫的表面被膜（SC）蛋白，这些蛋白被认为是松材线虫病发展的关键成分之一。这是首次使用蛋白质组学方法专注于 SC 蛋白并彻底鉴定植物寄生线虫的 SC 蛋白的报告。在这项研究中，比较了在真菌 Botrytis cinerea 和宿主松树苗上生长的松材线虫的 SC 蛋白谱。结果表明，在感染宿主松树期间，松材线虫 SC 蛋白的总量急剧增加。在真菌生长和宿主来源的松材线虫之间有 37 个蛋白条带表现出明显的数量差异，并用基质辅助激光解吸电离飞行时间质谱分析进行了鉴定。其中包括一些被认为参与宿主免疫反应的蛋白，例如活性氧（ROS）调节剂和 ROS 清除剂。这些结果可能表明松材线虫 SC 蛋白在调节或逃避宿主免疫反应中起关键作用。我们的数据为松材线虫病的发病机制和植物寄生线虫的 SC 蛋白的生物学作用提供了新的见解。
