Department of Otolaryngology / Head & Neck Surgery, Hadassah Hebrew-University Medical Center, Jerusalem 91120, Israel.
Immunol Invest. 2011;40(2):150-9. doi: 10.3109/08820139.2010.517589. Epub 2010 Nov 10.
The purpose of this study is to determine the expression of CD14 as a marker of the innate immunity in hypertrophic adenoids and tonsils. Twenty-four pediatric patients (age <12 years) with obstructive adenotonsillar hypertrophy, confirmed by sleep study were included in this study. Intensity and expression of positive CD14 infiltrating cells was assessed by immunohistochemistry in specific histologic areas. In tonsils, CD14 immunoreactivity was demonstrated in intraepithelial lymphocytes located in the basal layer of the stratified squamous mucoepithelium. CD14 expression was significantly higher in mucosal layers and inter-follicular areas of tonsils than adenoid tissues [(p < 0.001), (p = 0.021), respectively]. CD14 expression was significantly higher in the submucosal layers of adenoids than tonsil tissues (p = 0.002). Hypertrophic adenoids and tonsils from children with OSA are prominent sites of innate defense, with over expression of CD14. The enhanced expressions of CD14 cells in adenoids and tonsils may be an important factor for the development and persistence of adenoids and tonsils enlargement causing OSA in children. CD14 expression in adenoids and tonsils illustrates an important immunological sentinel function of the innate immunity of the upper airway.
本研究旨在确定 CD14 作为固有免疫标志物在上皮样和扁桃体组织增生中的表达。研究纳入了 24 名经睡眠研究证实患有阻塞性腺样体和扁桃体肥大的儿科患者（年龄<12 岁）。通过免疫组织化学在特定组织学区域评估阳性 CD14 浸润细胞的强度和表达。在扁桃体中，CD14 免疫反应性表现为位于复层鳞状黏膜上皮基底层的上皮内淋巴细胞。与腺样体组织相比，扁桃体组织的黏膜层和滤泡间区域的 CD14 表达明显更高[（p<0.001），（p=0.021）]。腺样体组织的黏膜下层中 CD14 的表达明显高于扁桃体组织（p=0.002）。患有 OSA 的儿童的肥大的腺样体和扁桃体是固有防御的显著部位，CD14 过度表达。腺样体和扁桃体中 CD14 细胞的增强表达可能是导致儿童 OSA 的腺样体和扁桃体增大发展和持续存在的重要因素。腺样体和扁桃体中的 CD14 表达表明了上呼吸道固有免疫的重要免疫监视功能。
