Montiel Daniel, Yates Emma V, Sun Li, Sampias Marissa M, Malona John, Sorensen Erik J, Yang Haw
Princeton University, Princeton, NJ, USA.
Methods Mol Biol. 2013;1025:237-50. doi: 10.1007/978-1-62703-462-3_18.
Silver and large gold nanoparticles are more efficient scatterers than smaller particles, which can be advantageous for a variety of single-particle-based sensing and spectroscopic applications. The increased susceptibility to surface oxidation and the larger surface area of these particles, however, present challenges to colloid stability and controllable bio-conjugation strategies. In this chapter, ligand syntheses and particle passivation procedures for yielding stable and bio-conjugatable colloids of silver and large gold nanoparticles are described.
相较于较小的粒子，银纳米粒子和大尺寸金纳米粒子是更高效的散射体，这对于各种基于单粒子的传感和光谱应用可能是有利的。然而，这些粒子对表面氧化的敏感性增加以及更大的表面积，给胶体稳定性和可控的生物共轭策略带来了挑战。在本章中，将描述用于制备稳定且可生物共轭的银纳米粒子和大尺寸金纳米粒子胶体的配体合成及粒子钝化程序。
