Ritchie Caroline M, Johnsen Kenneth R, Kiser John R, Antoku Yasuko, Dickson Robert M, Petty Jeffrey T
Department of Chemistry, Furman University, Greenville, South Carolina 29613.
J Phys Chem C Nanomater Interfaces. 2007 Jan 11;111(1):175-181. doi: 10.1021/jp0648487.
The reduction of silver cations bound to the oligonucleotide dC(12) was used to form silver nanoclusters. Mass spectra show that the oligonucleotides have 2-7 silver atoms that form multiple species, as evident from the number of transitions in the fluorescence and absorption spectra. The variations in the concentrations of the nanoclusters with time are attributed to the changing reducing capacity of the solution, and the formation of oxidized nanoclusters is proposed. Via mass spectrometry and circular dichroism spectroscopy, double-stranded structures with Ag(+)-mediated interactions between the bases are observed, but these structures are not maintained with the reduced nanoclusters. Through variations in the pH, the nanoclusters are shown to bind with the N3 of cytosine.
与寡核苷酸dC(12)结合的银阳离子的还原被用于形成银纳米簇。质谱表明，寡核苷酸含有2至7个银原子，这些银原子形成多种物种，这从荧光和吸收光谱中的跃迁数量可以明显看出。纳米簇浓度随时间的变化归因于溶液还原能力的改变，并提出了氧化纳米簇的形成。通过质谱和圆二色光谱法，观察到碱基之间存在Ag(+)介导相互作用的双链结构，但这些结构在还原后的纳米簇中无法维持。通过改变pH值，表明纳米簇与胞嘧啶的N3结合。
