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通过显微切割和DNA的序列非依赖性扩增对特定染色体区域的DNA进行分子克隆。

Molecular cloning of DNA from specific chromosomal regions by microdissection and sequence-independent amplification of DNA.

作者信息

Johnson D H

机构信息

Department of Biochemistry and Molecular Biology, University of Miami School of Medicine, Florida 33136.

出版信息

Genomics. 1990 Feb;6(2):243-51. doi: 10.1016/0888-7543(90)90563-a.

DOI:10.1016/0888-7543(90)90563-a
PMID:2106479
Abstract

A method that permits the in vitro amplification and cloning of DNA dissected from specific regions of a chromosome and does not require prior knowledge of the DNA sequence is described. DNA from several different chromosomal loci in the Drosophila melanogaster genome has been isolated by this method. Although the procedure was developed to permit the isolation of DNA sequences from serial sections of a single microdissected polytene chromosome, it should be useful for obtaining DNA clones from specific regions of the nonpolytene chromosomes of other organisms as well.

摘要

本文描述了一种方法，该方法可在体外扩增并克隆从染色体特定区域分离出的DNA，且无需事先了解DNA序列。通过此方法已从黑腹果蝇基因组的几个不同染色体位点分离出了DNA。尽管该程序是为从单个显微切割的多线染色体的连续切片中分离DNA序列而开发的，但它对于从其他生物体的非多线染色体的特定区域获得DNA克隆也应是有用的。

相似文献

1
Molecular cloning of DNA from specific chromosomal regions by microdissection and sequence-independent amplification of DNA.通过显微切割和DNA的序列非依赖性扩增对特定染色体区域的DNA进行分子克隆。
Genomics. 1990 Feb;6(2):243-51. doi: 10.1016/0888-7543(90)90563-a.
2
Cloning regions of the Drosophila genome by microdissection of polytene chromosome DNA and PCR with nonspecific primer.通过多线染色体DNA的显微切割和用非特异性引物进行聚合酶链式反应克隆果蝇基因组区域。
Nucleic Acids Res. 1990 Feb 11;18(3):599-603. doi: 10.1093/nar/18.3.599.
3
Telomeric sequences derived from laser-microdissected polytene chromosomes.源自激光显微切割多线染色体的端粒序列。
Chromosoma. 1989 Nov;98(5):351-7. doi: 10.1007/BF00292388.
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Uneven distribution of cloned transcribed DNA sequences in polytene chromosomes of Drosophila melanogaster.黑腹果蝇多线染色体中克隆转录DNA序列的不均匀分布。
Cell Differ. 1986 Mar;18(2):145-9. doi: 10.1016/0045-6039(86)90009-6.
5
Cloning defined regions of the human genome by microdissection of banded chromosomes and enzymatic amplification.通过对带型染色体进行显微切割和酶促扩增来克隆人类基因组的特定区域。
Nature. 1989 Mar 23;338(6213):348-50. doi: 10.1038/338348a0.
6
PCR amplification of DNA microdissected from a single polytene chromosome band: a comparison with conventional microcloning.从单个多线染色体带显微切割的DNA的PCR扩增：与传统微克隆的比较。
Nucleic Acids Res. 1989 Nov 25;17(22):9027-37. doi: 10.1093/nar/17.22.9027.
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[Cloning and analysis of DNA from interband regions 85D9/D10 and 86B4/B6 of Drosophila melanogaster polytene chromosomes].[黑腹果蝇多线染色体85D9/D10和86B4/B6间带区DNA的克隆与分析]
Genetika. 1998 Aug;34(8):1081-9.
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Towards a physical map of the Drosophila melanogaster genome: mapping of cosmid clones within defined genomic divisions.迈向黑腹果蝇基因组物理图谱：在特定基因组区域内对黏粒克隆进行定位
Nucleic Acids Res. 1990 Nov 11;18(21):6261-70. doi: 10.1093/nar/18.21.6261.
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Microdissection and cloning of DNA from a specific region of Drosophila melanogaster polytene chromosomes.从黑腹果蝇多线染色体特定区域进行DNA的显微切割与克隆。
Chromosoma. 1981;82(2):205-16. doi: 10.1007/BF00286105.
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Three euchromatic DNA sequences under-replicated in polytene chromosomes of Drosophila are localized in constrictions and ectopic fibers.在果蝇多线染色体中复制不足的三个常染色质DNA序列位于缢痕和异位纤维中。
Chromosoma. 1987;95(4):227-35. doi: 10.1007/BF00294779.

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Theor Appl Genet. 1996 Sep;93(4):477-84. doi: 10.1007/BF00417938.
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The development of chromosome microdissection and microcloning technique and its applications in genomic research.
染色体显微切割和微克隆技术的发展及其在基因组研究中的应用。
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Disclosure of five breakpoints in a complex chromosome rearrangement by microdissection and FISH.通过显微切割和荧光原位杂交技术揭示复杂染色体重排中的五个断点
J Med Genet. 1996 Jul;33(7):562-6. doi: 10.1136/jmg.33.7.562.
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Chromosome 'painting' in plants - a feasible technique?植物中的染色体“描绘”——一种可行的技术？
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Libraries for each human chromosome, constructed from sorter-enriched chromosomes by using linker-adaptor PCR.通过使用接头衔接子PCR从分选富集的染色体构建的每个人类染色体文库。
Am J Hum Genet. 1993 Mar;52(3):586-97.
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