对含有封闭N端氨基酸（N-乙酰丝氨酸或N-乙酰苏氨酸）的肽和蛋白质进行测序。
Sequencing of peptides and proteins with blocked N-terminal amino acids: N-acetylserine or N-acetylthreonine.
作者信息
Wellner D, Panneerselvam C, Horecker B L
机构信息
Department of Biochemistry, Cornell University Medical College, New York, NY 10021.
出版信息
Proc Natl Acad Sci U S A. 1990 Mar;87(5):1947-9. doi: 10.1073/pnas.87.5.1947.
Abstract
Many proteins cannot be directly sequenced by Edman degradation because they have a blocked N-terminal residue. A method is presented for deblocking such proteins when the N-terminal residue is N-acetylserine (which occurs frequently in eukaryotic proteins) or N-acetylthreonine. The method has been applied successfully to the determination of the N-terminal amino acid sequence of human, bovine, and rat parathymosins. Prothymosin alpha and other blocked proteins and peptides were also readily deblocked and sequenced by this procedure. It is proposed that the mechanism of the deblocking reaction involves an acid-catalyzed N----O shift of the acetyl group followed by a beta-elimination.
摘要
许多蛋白质无法通过埃德曼降解法直接测序，因为它们的N端残基被封闭。本文介绍了一种对N端残基为N - 乙酰丝氨酸（在真核蛋白质中频繁出现）或N - 乙酰苏氨酸的此类蛋白质进行去封闭的方法。该方法已成功应用于测定人、牛和大鼠甲状旁胸腺素的N端氨基酸序列。胸腺素α原和其他封闭的蛋白质及肽也可通过此程序轻松去封闭并测序。有人提出，去封闭反应的机制涉及乙酰基的酸催化N→O迁移，随后进行β消除。
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本文引用的文献
1
Amino-acid sequence of horse heart cytochrome c.马心脏细胞色素c的氨基酸序列。
Nature. 1961 Dec 23;192:1125-7. doi: 10.1038/1921125a0.
2
Isolation of acetylpeptide from enzymic digests of TMV-protein.从烟草花叶病毒蛋白的酶解消化物中分离乙酰肽。
Biochim Biophys Acta. 1958 Apr;28(1):184-91. doi: 10.1016/0006-3002(58)90445-1.
3
Complete amino acid sequence of bovine thymosin beta 4: a thymic hormone that induces terminal deoxynucleotidyl transferase activity in thymocyte populations.牛胸腺素β4的完整氨基酸序列：一种能诱导胸腺细胞群体中末端脱氧核苷酸转移酶活性的胸腺激素。
Proc Natl Acad Sci U S A. 1981 Feb;78(2):1162-6. doi: 10.1073/pnas.78.2.1162.
4
Pyrrolidonyl peptidase. An enzyme for selective removal of pyrrolidonecarboxylic acid residues from polypeptides.吡咯烷酮肽酶。一种用于从多肽中选择性去除吡咯烷酮羧酸残基的酶。
Biochemistry. 1968 Feb;7(2):516-21. doi: 10.1021/bi00842a005.
5
Amino acid sequence of the peptide released from bovine factor XIII following activation by thrombin.凝血酶激活后从牛因子XIII释放的肽的氨基酸序列。
Biochem Biophys Res Commun. 1974 May 7;58(1):250-6. doi: 10.1016/0006-291x(74)90919-x.
6
The N- and c-terminal amino acid sequences of the heavy chain from a pathological human immunoglobulin IgG.来自病理性人类免疫球蛋白IgG的重链的N端和C端氨基酸序列。
Biochem J. 1966 May;99(2):356-66. doi: 10.1042/bj0990356.
7
Structures of N-terminally acetylated proteins.
Eur J Biochem. 1985 Nov 4;152(3):523-7. doi: 10.1111/j.1432-1033.1985.tb09227.x.
8
Simultaneous isolation and determination of prothymosin alpha, parathymosin alpha, thymosin beta 4, and thymosin beta 10.同时分离并测定前胸腺素α、副胸腺素α、胸腺素β4和胸腺素β10。
Anal Biochem. 1985 Feb 1;144(2):436-40. doi: 10.1016/0003-2697(85)90138-1.
9
Studies on N alpha-acylated proteins: the N-terminal sequences of two muscle enolases.
Biosci Rep. 1985 Oct-Nov;5(10-11):847-54. doi: 10.1007/BF01119896.
10
Complete amino acid sequence of human hemopexin, the heme-binding protein of serum.人血清血红素结合蛋白——血红素结合蛋白的完整氨基酸序列
Proc Natl Acad Sci U S A. 1985 Jan;82(1):73-7. doi: 10.1073/pnas.82.1.73.
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