• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大麦真菌病原体禾谷镰刀菌 f. teres 的第一个基因组组装。

A first genome assembly of the barley fungal pathogen Pyrenophora teres f. teres.

机构信息

Department of Environment and Agriculture, Curtin University, Kent Street, Bentley, Perth, Western Australia 6102, Australia.

出版信息

Genome Biol. 2010;11(11):R109. doi: 10.1186/gb-2010-11-11-r109. Epub 2010 Nov 10.

DOI:10.1186/gb-2010-11-11-r109
PMID:21067574
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3156948/
Abstract

BACKGROUND

Pyrenophora teres f. teres is a necrotrophic fungal pathogen and the cause of one of barley's most important diseases, net form of net blotch. Here we report the first genome assembly for this species based solely on short Solexa sequencing reads of isolate 0-1. The assembly was validated by comparison to BAC sequences, ESTs, orthologous genes and by PCR, and complemented by cytogenetic karyotyping and the first genome-wide genetic map for P. teres f. teres.

RESULTS

The total assembly was 41.95 Mbp and contains 11,799 gene models of 50 amino acids or more. Comparison against two sequenced BACs showed that complex regions with a high GC content assembled effectively. Electrophoretic karyotyping showed distinct chromosomal polymorphisms between isolates 0-1 and 15A, and cytological karyotyping confirmed the presence of at least nine chromosomes. The genetic map spans 2477.7 cM and is composed of 243 markers in 25 linkage groups, and incorporates simple sequence repeat markers developed from the assembly. Among predicted genes, non-ribosomal peptide synthetases and efflux pumps in particular appear to have undergone a P. teres f. teres-specific expansion of non-orthologous gene families.

CONCLUSIONS

This study demonstrates that paired-end Solexa sequencing can successfully capture coding regions of a filamentous fungal genome. The assembly contains a plethora of predicted genes that have been implicated in a necrotrophic lifestyle and pathogenicity and presents a significant resource for examining the bases for P. teres f. teres pathogenicity.

摘要

背景

层出镰刀菌（Pyrenophora teres f. teres）是一种坏死型真菌病原体，也是大麦最重要病害之一——网斑病的病原体。本文报道了首个基于 0-1 分离株的 Solexa 短测序reads 的该物种基因组序列，该序列通过与 BAC 序列、EST 序列、直系同源基因和 PCR 比较进行了验证，并通过细胞遗传学核型分析和首个层出镰刀菌全基因组遗传图谱进行了补充。

结果

总组装大小为 41.95 Mbp，包含 11799 个 50 个氨基酸或以上的基因模型。与两个已测序 BAC 的比较表明，高 GC 含量的复杂区域能有效组装。电泳核型分析显示，0-1 和 15A 分离株之间存在明显的染色体多态性，细胞遗传学核型分析证实至少存在 9 条染色体。遗传图谱跨越 2477.7 cM，由 25 个连锁群中的 243 个标记组成，并整合了来自组装的简单序列重复标记。在预测基因中，非核糖体肽合成酶和外排泵尤其表现出层出镰刀菌特有的非直系同源基因家族的扩张。

结论

本研究表明，Solexa 测序可以成功捕获丝状真菌基因组的编码区。该组装包含大量与坏死型生活方式和致病性相关的预测基因，为研究层出镰刀菌致病性的基础提供了重要资源。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/ba5f4312103f/gb-2010-11-11-r109-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/29e5ea95aa09/gb-2010-11-11-r109-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/7f8c40acc863/gb-2010-11-11-r109-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/72b412073493/gb-2010-11-11-r109-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/f70499cd1baf/gb-2010-11-11-r109-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/b50d6e3c4641/gb-2010-11-11-r109-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/ba5f4312103f/gb-2010-11-11-r109-6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/29e5ea95aa09/gb-2010-11-11-r109-1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/7f8c40acc863/gb-2010-11-11-r109-2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/72b412073493/gb-2010-11-11-r109-3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/f70499cd1baf/gb-2010-11-11-r109-4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/b50d6e3c4641/gb-2010-11-11-r109-5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a67a/3156948/ba5f4312103f/gb-2010-11-11-r109-6.jpg

相似文献

1
A first genome assembly of the barley fungal pathogen Pyrenophora teres f. teres.大麦真菌病原体禾谷镰刀菌 f. teres 的第一个基因组组装。
Genome Biol. 2010;11(11):R109. doi: 10.1186/gb-2010-11-11-r109. Epub 2010 Nov 10.
2
Reference Assembly and Annotation of the f. Isolate 0-1.f.分离株0-1的参考组装与注释
G3 (Bethesda). 2018 Jan 4;8(1):1-8. doi: 10.1534/g3.117.300196.
3
Evaluation of a Pyrenophora teres f. teres mapping population reveals multiple independent interactions with a region of barley chromosome 6H.对网斑病菌条形柄锈菌变种的一个作图群体的评估揭示了与大麦6H染色体区域的多个独立互作。
Fungal Genet Biol. 2014 Sep;70:104-12. doi: 10.1016/j.fgb.2014.07.012. Epub 2014 Aug 2.
4
Pyrenophora teres: profile of an increasingly damaging barley pathogen.禾谷丝核菌：一种危害性日益增加的大麦病原菌。
Mol Plant Pathol. 2011 Jan;12(1):1-19. doi: 10.1111/j.1364-3703.2010.00649.x.
5
Genetic analysis of virulence in the Pyrenophora teres f. teres population BB25×FGOH04Ptt-21.在 Pyrenophora teres f. teres 群体 BB25×FGOH04Ptt-21 中进行毒力的遗传分析。
Fungal Genet Biol. 2017 Oct;107:12-19. doi: 10.1016/j.fgb.2017.07.003. Epub 2017 Jul 17.
6
Association mapping utilizing diverse barley lines reveals net form net blotch seedling resistance/susceptibility loci.利用不同的大麦品系进行关联作图揭示了网斑病苗期抗性/感病的位点。
Theor Appl Genet. 2017 May;130(5):915-927. doi: 10.1007/s00122-017-2860-1. Epub 2017 Feb 9.
7
Mating type locus-specific polymerase chain reaction markers for differentiation of Pyrenophora teres f. teres and P. teres f. maculata, the causal agents of barley net blotch.区分禾谷丝核菌禾谷变种和禾谷丝核菌叶斑变种，即大麦网斑病病原菌的交配型座特异性聚合酶链反应标记。
Phytopathology. 2010 Dec;100(12):1298-306. doi: 10.1094/PHYTO-05-10-0135.
8
Four Reference Quality Genome Assemblies of f. : A Resource for Studying the Barley Spot Form Net Blotch Interaction.四个 f. 的参考质量基因组组装：研究大麦斑点形成网斑病互作的资源。
Mol Plant Microbe Interact. 2021 Jan;34(1):135-139. doi: 10.1094/MPMI-08-20-0228-A. Epub 2020 Dec 2.
9
A Comparative Genomic Analysis of the Barley Pathogen f. Identifies Subtelomeric Regions as Drivers of Virulence.大麦病原菌 f 的比较基因组分析确定端粒下区域为毒力驱动因素。
Mol Plant Microbe Interact. 2020 Feb;33(2):173-188. doi: 10.1094/MPMI-05-19-0128-R. Epub 2019 Nov 13.
10
Fine Mapping of the Barley Chromosome 6H Net Form Net Blotch Susceptibility Locus.大麦6H染色体网形网斑病感病基因座的精细定位
G3 (Bethesda). 2016 Jul 7;6(7):1809-18. doi: 10.1534/g3.116.028902.

引用本文的文献

1
Microbial decay of wooden structures: actors, activities and means of protection.木结构的微生物腐朽：作用因素、活动及防护手段
Appl Microbiol Biotechnol. 2025 Mar 5;109(1):59. doi: 10.1007/s00253-025-13443-z.
2
What are the 100 most cited fungal genera?被引用次数最多的100个真菌属有哪些？
Stud Mycol. 2024 Jul;108:1-411. doi: 10.3114/sim.2024.108.01. Epub 2024 Jul 15.
3
Stepwise recombination suppression around the mating-type locus in an ascomycete fungus with self-fertile spores.在一个具有可育孢子的子囊菌真菌中，交配型基因座周围的逐步重组抑制。

本文引用的文献

1
Pyrenophora teres: profile of an increasingly damaging barley pathogen.禾谷丝核菌：一种危害性日益增加的大麦病原菌。
Mol Plant Pathol. 2011 Jan;12(1):1-19. doi: 10.1111/j.1364-3703.2010.00649.x.
2
Host-selective toxins, Ptr ToxA and Ptr ToxB, as necrotrophic effectors in the Pyrenophora tritici-repentis-wheat interaction.寄主选择性毒素 Ptr ToxA 和 Ptr ToxB 作为禾谷丝核菌-小麦互作中的坏死型效应因子。
New Phytol. 2010 Sep;187(4):911-9. doi: 10.1111/j.1469-8137.2010.03362.x. Epub 2010 Jul 14.
3
Pathogenicity genes of phytopathogenic fungi.
PLoS Genet. 2023 Feb 10;19(2):e1010347. doi: 10.1371/journal.pgen.1010347. eCollection 2023 Feb.
4
IMA genome‑F17 : Draft genome sequences of an Armillaria species from Zimbabwe, Ceratocystis colombiana, Elsinoë necatrix, Rosellinia necatrix, two genomes of Sclerotinia minor, short‑read genome assemblies and annotations of four Pyrenophora teres isolates from barley grass, and a long-read genome assembly of Cercospora zeina.IMA基因组-F17：来自津巴布韦的一种蜜环菌、哥伦比亚长喙壳菌、毁灭炭疽菌、寄生白纹羽病菌、两种小核盘菌基因组、来自大麦草的4个网斑病菌分离株的短读长基因组组装和注释，以及玉蜀黍尾孢菌的长读长基因组组装。
IMA Fungus. 2022 Nov 21;13(1):19. doi: 10.1186/s43008-022-00104-3.
5
The First Telomere-to-Telomere Chromosome-Level Genome Assembly of Causing Sugarcane Leaf Blight.引起甘蔗叶枯病的首个端粒到端粒的染色体水平基因组组装。
J Fungi (Basel). 2022 Oct 16;8(10):1088. doi: 10.3390/jof8101088.
6
Endophytic fungi from kale ( var. ) modify roots-glucosinolate profile and promote plant growth in cultivated species. First description of .羽衣甘蓝（变种）内生真菌改变根中硫代葡萄糖苷谱并促进栽培物种的植物生长。首次描述……
Front Microbiol. 2022 Oct 5;13:981507. doi: 10.3389/fmicb.2022.981507. eCollection 2022.
7
Genome Analyses of Two Blueberry Pathogens: CAA958 and CBS 160.32.两种蓝莓病原体的基因组分析：CAA958和CBS 160.32
J Fungi (Basel). 2022 Jul 29;8(8):804. doi: 10.3390/jof8080804.
8
IMA Genome - F15 : Draft genome assembly of Fusarium pilosicola, Meredithiella fracta, Niebla homalea, Pyrenophora teres hybrid WAC10721, and Teratosphaeria viscida.IMA基因组 - F15：毛柄镰刀菌、裂褶菌、平截条孢地衣、网斑病菌杂交种WAC10721和粘性球腔菌的基因组草图组装
IMA Fungus. 2021 Oct 13;12(1):30. doi: 10.1186/s43008-021-00077-9.
9
An optimized sporulation method for the wheat fungal pathogen Pyrenophora tritici-repentis.一种针对小麦真菌病原体小麦根腐离蠕孢的优化产孢方法。
Plant Methods. 2021 May 19;17(1):52. doi: 10.1186/s13007-021-00751-4.
10
: Taxonomy, Morphology, Interaction With Barley, and Mode of Control.分类学、形态学、与大麦的相互作用及防治方式
Front Plant Sci. 2021 Apr 6;12:614951. doi: 10.3389/fpls.2021.614951. eCollection 2021.
植物病原真菌的致病性基因。
Mol Plant Pathol. 2001 Jul 1;2(4):241-55. doi: 10.1046/j.1464-6722.2001.00070.x.
4
Ferricrocin synthesis in Magnaporthe grisea and its role in pathogenicity in rice.稻瘟病菌中血褐菌素的合成及其在水稻致病性中的作用。
Mol Plant Pathol. 2007 Mar;8(2):163-72. doi: 10.1111/j.1364-3703.2007.00380.x.
5
De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis.从头组装 40Mb 真核生物基因组的短序列读取：Sordaria macrospora，一种真菌形态发生的模式生物。
PLoS Genet. 2010 Apr 8;6(4):e1000891. doi: 10.1371/journal.pgen.1000891.
6
Comparative genomics reveals mobile pathogenicity chromosomes in Fusarium.比较基因组学揭示了镰刀菌中可移动的致病性染色体。
Nature. 2010 Mar 18;464(7287):367-73. doi: 10.1038/nature08850.
7
BEDTools: a flexible suite of utilities for comparing genomic features.BEDTools：一套灵活的基因组特征比较工具套件。
Bioinformatics. 2010 Mar 15;26(6):841-2. doi: 10.1093/bioinformatics/btq033. Epub 2010 Jan 28.
8
SnTox3 acts in effector triggered susceptibility to induce disease on wheat carrying the Snn3 gene.SnTox3在效应子触发的易感性中发挥作用，从而在携带Snn3基因的小麦上引发病害。
PLoS Pathog. 2009 Sep;5(9):e1000581. doi: 10.1371/journal.ppat.1000581. Epub 2009 Sep 18.
9
Deep proteogenomics; high throughput gene validation by multidimensional liquid chromatography and mass spectrometry of proteins from the fungal wheat pathogen Stagonospora nodorum.深度蛋白基因组学；通过多维液相色谱和真菌小麦病原体禾谷核腔菌蛋白的质谱对基因进行高通量验证。
BMC Bioinformatics. 2009 Sep 22;10:301. doi: 10.1186/1471-2105-10-301.
10
Cytological and electrophoretic karyotyping of the chestnut blight fungus Cryphonectria parasitica.栗疫病菌寄生隐孢壳菌的细胞学和电泳核型分析
Fungal Genet Biol. 2009 Apr;46(4):342-51. doi: 10.1016/j.fgb.2009.01.005. Epub 2009 Feb 6.