Xu Fu, Li Xiuxiu, Ren Hui, Zeng Rensen, Wang Zhoutao, Hu Hongli, Bao Jiandong, Que Youxiong
Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture and Rural Affairs, Fuzhou 350002, China.
College of Plant Protection, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
J Fungi (Basel). 2022 Oct 16;8(10):1088. doi: 10.3390/jof8101088.
The sexual morph Yen and Chi and its asexual morph W. H. Hsieh is an important necrotrophic fungal phytopathogen, which causes sugarcane leaf blight, resulting in loss of cane tonnage and sucrose in susceptible sugarcane varieties. Decoding the genome and understanding of the basis of virulence is vitally important for devising effective disease control strategies. Here, we present a 38.25-Mb high-quality genome assembly of strain StFZ01, denovo assembled with 10.19 Gb Nanopore sequencing long reads (267×) and 3.82 Gb Illumina short reads (100×). The genome assembly consists of 12 contigs with N50 of 2.86 Mb of which 5 belong to the telomere to telomere (T2T) chromosome. It contains 13.20% repeat sequences, 12,543 proteins, and 12,206 protein-coding genes with the BUSCO completeness 99.18% at fungi ( = 758) and 99.87% at ascomycota ( = 1706), indicating the high accuracy and completeness of our gene annotations. The virulence analysis in silico revealed the presence of 2379 PHIs, 599 CAZys, 248 membrane transport proteins, 191 cytochrome P450 enzymes, 609 putative secreted proteins, and 333 effectors in the StFZ01 genome. The genomic resources presented here will not only be helpful for development of specific molecular marker and diagnosis technique, population genetics, molecular taxonomy, and disease managements, it can also provide a significant precise genomic reference for investigating the ascomycetous genome, the necrotrophic lifestyle, and pathogenicity in the future.
有性型Yen和Chi及其无性型W. H. Hsieh是一种重要的坏死营养型真菌植物病原体，可导致甘蔗叶枯病，使易感甘蔗品种的甘蔗产量和蔗糖含量降低。解码基因组并了解其毒力基础对于制定有效的病害控制策略至关重要。在此，我们展示了菌株StFZ01的38.25 Mb高质量基因组组装，该组装利用10.19 Gb纳米孔测序长读段（约267×）和3.82 Gb Illumina短读段（约100×）进行从头组装。基因组组装由12个重叠群组成，N50为2.86 Mb，其中5个属于端粒到端粒（T2T）染色体。它包含13.20%的重复序列、12,543个蛋白质以及12,206个蛋白质编码基因，在真菌（= 758）中的BUSCO完整性为99.18%，在子囊菌门（= 1706）中的BUSCO完整性为99.87%，表明我们的基因注释具有很高的准确性和完整性。计算机毒力分析显示，StFZ01基因组中存在2379个植物-病原体互作基因、599个碳水化合物活性酶、248个膜转运蛋白、191个细胞色素P450酶、609个推定的分泌蛋白和333个效应子。本文提供的基因组资源不仅有助于开发特定的分子标记和诊断技术、群体遗传学、分子分类学以及病害管理，还可为未来研究子囊菌基因组、坏死营养型生活方式和致病性提供重要的精确基因组参考。
