Molecular and Cellular Biology Laboratory, National Institute for Research in Reproductive Health, Parel, Mumbai, India.
Fertil Steril. 2011 Mar 15;95(4):1278-83. doi: 10.1016/j.fertnstert.2010.09.045. Epub 2010 Nov 10.
To evaluate the effects of decidua-derived factors on trophoblast invasion.
Experimental study.
Research institute.
PATIENT(S): In vitro decidualized human endometrial cells, trophoblast cell lines JEG-3, and ACH-3P.
INTERVENTION(S): The effect of decidual conditioned medium (DCM) on the invasion of trophoblast cells lines via JEG-3 and ACH-3P was investigated using a Matrigel invasion assay. The changes in expression of matrix metalloproteinases (MMPs), tissue inhibitors of metalloproteinases (TIMPs) and integrins in response to DCM in the trophoblast cells was also evaluated.
MAIN OUTCOME MEASURE(S): Response of the trophoblast cells to the conditioned medium from decidual cells in terms of their invasive capability, and expression on invasion related molecules was measured.
RESULT(S): DCM increased the invasion of both the cell lines by approximately 1.8-2.2-fold, compared with control condition medium. The increase in invasion was associated with elevated levels of MMP2, MMP3, and MMP9 mRNA and increased activity of MMP2 and MMP9 in DCM-treated ACH-3P, but not JEG-3 cells. DCM treatment led to a reduction in TIMP1 and TIMP3 and increased TIMP2 mRNA in JEG-3, cells but not ACH-3P cells. Compared with CCM-treated controls, DCM treatment led to a significant increase in the mRNA expression of integrin α5 and α6, but not integrin αV subunit in both cell lines.
CONCLUSION(S): Decidua-derived factors increase the invasiveness of trophoblast cell lines and alter the expression of integrins, MMPs, and TIMPs.
评估蜕膜源性因子对滋养细胞侵袭的影响。
实验研究。
研究所。
体外蜕膜化的人子宫内膜细胞、滋养细胞系 JEG-3 和 ACH-3P。
通过 Matrigel 侵袭试验研究蜕膜条件培养基（DCM）对滋养细胞系 JEG-3 和 ACH-3P 侵袭的影响。还评估了滋养细胞对 DCM 反应中基质金属蛋白酶（MMPs）、金属蛋白酶组织抑制剂（TIMPs）和整合素表达的变化。
滋养细胞对蜕膜细胞条件培养基反应的侵袭能力及其侵袭相关分子的表达。
与对照条件培养基相比，DCM 使两种细胞系的侵袭率增加了约 1.8-2.2 倍。侵袭增加与 MMP2、MMP3 和 MMP9 mRNA 水平升高以及 MMP2 和 MMP9 在 DCM 处理的 ACH-3P 细胞中的活性增加有关，但在 JEG-3 细胞中则不然。DCM 处理导致 JEG-3 细胞中 TIMP1 和 TIMP3 减少和 TIMP2 mRNA 增加，但在 ACH-3P 细胞中则不然。与 CCM 处理的对照组相比，DCM 处理导致两种细胞系中整合素 α5 和 α6 的 mRNA 表达显著增加，但整合素 αV 亚基则不然。
蜕膜源性因子增加滋养细胞系的侵袭性，并改变整合素、MMPs 和 TIMPs 的表达。
