Centre for Cell Engineering, University of Glasgow, Glasgow G12 8QQ, UK.
Small. 2010 Dec 6;6(23):2755-61. doi: 10.1002/smll.201000193.
A biodegradable substrate with a regular array of nanopillars fabricated by electron-beam lithography and hot embossing is used to address the mechanisms of nanotopographical control of cell behavior. Two different cell lines cultured on the nanopillars show striking differences in cell coverage. These changes are topography- and cell-dependent, and are not mediated by air bubbles trapped on the nanopattern. For the first time, a strong cell-selective effect of the same nanotopography has been clearly demonstrated on a large area; while fibroblast proliferation is inhibited, endothelial cell spreading is visibly enhanced. The reduced fibroblast proliferation indicates that a reduction of available surface area induced by nanotopography might be the main factor affecting cell growth on nanopatterns. The results presented herein pave the way towards the development of permanent vascular replacements, where non-adhesive, inert, surfaces will induce rapid in situ endothelialization to reduce thrombosis and occlusion.
采用电子束光刻和热压印技术制作具有规则纳米柱阵列的生物可降解基底，用于研究纳米形貌控制细胞行为的机制。两种不同的细胞系在纳米柱上的培养表现出明显的细胞覆盖率差异。这些变化与形貌和细胞有关，而不是由纳米图案上捕获的气泡介导的。首次在大面积上清楚地证明了相同纳米形貌的强烈细胞选择性效应；虽然成纤维细胞增殖受到抑制，但内皮细胞的铺展明显增强。成纤维细胞增殖减少表明，纳米形貌引起的可用表面积减少可能是影响细胞在纳米图案上生长的主要因素。本文的结果为开发永久性血管替代品铺平了道路，其中非粘附、惰性表面将诱导快速原位内皮化，以减少血栓形成和闭塞。
