Schieber Romain, Mas-Moruno Carlos, Lasserre Federico, Roa Joan Josep, Ginebra Maria-Pau, Mücklich Frank, Pegueroles Marta
Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Barcelona East School of Engineering (EEBE), Universitat Politècnica de Catalunya (UPC), Av. Eduard Maristany, 10-14, 08019 Barcelona, Spain.
Barcelona Research Center in Multiscale Science and Engineering, Universitat Politècnica de Catalunya (UPC), 08019 Barcelona, Spain.
Nanomaterials (Basel). 2022 Apr 5;12(7):1217. doi: 10.3390/nano12071217.
Endothelial coverage of an exposed cardiovascular stent surface leads to the occurrence of restenosis and late-stent thrombosis several months after implantation. To overcome this difficulty, modification of stent surfaces with topographical or biochemical features may be performed to increase endothelial cells' (ECs) adhesion and/or migration. This work combines both strategies on cobalt-chromium (CoCr) alloy and studies the potential synergistic effect of linear patterned surfaces that are obtained by direct laser interference patterning (DLIP), coupled with the use of Arg-Gly-Asp (RGD) and Tyr-Ile-Gly-Ser-Arg (YIGSR) peptides. An extensive characterization of the modified surfaces was performed by using AFM, XPS, surface charge, electrochemical analysis and fluorescent methods. The biological response was studied in terms of EC adhesion, migration and proliferation assays. CoCr surfaces were successfully patterned with a periodicity of 10 µm and two different depths, D (≈79 and 762 nm). RGD and YIGSR were immobilized on the surfaces by CPTES silanization. Early EC adhesion was increased on the peptide-functionalized surfaces, especially for YIGSR compared to RGD. High-depth patterns generated 80% of ECs' alignment within the topographical lines and enhanced EC migration. It is noteworthy that the combined use of the two strategies synergistically accelerated the ECs' migration and proliferation, proving the potential of this strategy to enhance stent endothelialization.
暴露的心血管支架表面的内皮覆盖会导致植入后数月出现再狭窄和晚期支架血栓形成。为克服这一难题，可对支架表面进行具有拓扑或生化特征的修饰，以增加内皮细胞（ECs）的黏附及/或迁移。本研究将这两种策略结合应用于钴铬（CoCr）合金，并研究通过直接激光干涉图案化（DLIP）获得的线性图案化表面与使用精氨酸-甘氨酸-天冬氨酸（RGD）和酪氨酸-异亮氨酸-甘氨酸-丝氨酸-精氨酸（YIGSR）肽的潜在协同效应。通过原子力显微镜（AFM）、X射线光电子能谱（XPS）、表面电荷、电化学分析和荧光方法对修饰后的表面进行了广泛表征。从ECs的黏附、迁移和增殖试验方面研究了生物学反应。CoCr表面成功地形成了周期为10 µm且具有两种不同深度D（≈79和762 nm）的图案。RGD和YIGSR通过3-（三甲氧基硅基）丙基异氰酸酯（CPTES）硅烷化固定在表面。与RGD相比，肽功能化表面上早期ECs黏附增加，尤其是YIGSR。高深度图案使80%的ECs沿拓扑线排列，并增强了ECs迁移。值得注意的是，两种策略的联合使用协同加速了ECs的迁移和增殖，证明了该策略在增强支架内皮化方面的潜力。
