Tamura-Nishimura M, Sasakawa S
Japanese Red Cross, Central Blood Center, Tokyo.
FEBS Lett. 1990 Feb 26;261(2):343-6. doi: 10.1016/0014-5793(90)80587-9.
Transcription of human interferon (IFN) gamma gene is induced in human peripheral lymphocyte nylon-nonadherent cells (NNA cells) by double strand RNA poly I:poly C [(1985) J. Interferon Res. 5, 77-84]. In this report, the necessity of de novo protein synthesis in an early stage of IFN gamma gene expression is described. For induction of IFN gamma gene expression, only initial 4 h treatment of poly I:poly C to NNA cells is sufficient. Addition of inhibitor of protein synthesis, cycloheximide (CHX), at an early stage of induction periods (0-4 h) inhibits the IFN gamma induction by poly I:poly C. Cell free translation assay using RNAs isolated from NNA cells which are induced by poly I:poly C in the presence of CHX reveals that in these RNAs, IFN gamma mRNA does not exist. These results demonstrate that CHX inhibits de novo synthesis of a certain protein (or proteins) and for lack of the protein(s), IFN gamma mRNA cannot be transcribed. The evidence is also described in this report which suggests that the essential protein(s) might be that (those) involved in protein kinase C (pkC) activation.
双链RNA多聚肌苷酸：多聚胞嘧啶核苷酸[（1985年）《干扰素研究杂志》5，77 - 84]可诱导人外周淋巴细胞尼龙非黏附细胞（NNA细胞）中人类干扰素（IFN）γ基因的转录。在本报告中，描述了IFNγ基因表达早期阶段从头合成蛋白质的必要性。为诱导IFNγ基因表达，仅最初4小时用多聚肌苷酸：多聚胞嘧啶核苷酸处理NNA细胞就足够了。在诱导期早期（0 - 4小时）添加蛋白质合成抑制剂环己酰亚胺（CHX）可抑制多聚肌苷酸：多聚胞嘧啶核苷酸诱导的IFNγ产生。使用从在CHX存在下被多聚肌苷酸：多聚胞嘧啶核苷酸诱导的NNA细胞中分离的RNA进行无细胞翻译试验表明，在这些RNA中不存在IFNγ mRNA。这些结果表明，CHX抑制某种蛋白质的从头合成，并且由于缺乏该蛋白质，IFNγ mRNA无法转录。本报告中还描述了证据，提示必需的蛋白质可能是参与蛋白激酶C（PKC）激活的蛋白质。
