针对 hilA、agfA、spvC 和 sef 基因的沙门氏菌株的基于 DNA 的诊断检测。
DNA-based diagnostic tests for Salmonella strains targeting hilA, agfA, spvC and sef genes.
机构信息
Babeş-Bolyai University, Faculty of Biology and Geology, 400006-Cluj-Napoca, Romania.
出版信息
J Environ Manage. 2012 Mar;95 Suppl:S15-8. doi: 10.1016/j.jenvman.2010.07.027. Epub 2010 Nov 10.
The goal of this study was to evaluate the suitability of the hilA, agfA, spvC and sef genes amplification by PCR as a method for detection of Salmonella strains. Twenty nine isolates of Salmonella spp. including 6 different serotypes were analyzed in this study. The bacteria were isolated between 2005 and 2007 and serotyped at the Clinical Hospital of Infectious Disease, Cluj-Napoca. Ten non-Salmonella strains were also tested by the same procedure. We used a direct PCR technique, DNA extraction had been skipped and the bacterial cell wall denaturated in the first step of the reaction. All Salmonella strains gave positive results by the PCR amplification of hilA gene. The utilization of the sef, and spvC genes or spvC and agfA genes in a multiplex PCR provides a valuable diagnostic tool for Salmonella enteritidis strains.
本研究的目的是评估通过 PCR 扩增 hilA、agfA、spvC 和 sef 基因作为检测沙门氏菌菌株的方法的适用性。在这项研究中分析了 29 株沙门氏菌属，包括 6 种不同的血清型。这些细菌是在 2005 年至 2007 年之间在克卢日-纳波卡传染病临床医院分离出来的，并进行了血清分型。同样的程序还测试了 10 株非沙门氏菌菌株。我们使用了一种直接 PCR 技术，省略了 DNA 提取步骤，并在反应的第一步使细菌细胞壁变性。所有沙门氏菌菌株通过 hilA 基因的 PCR 扩增都得到了阳性结果。sef 和 spvC 基因或 spvC 和 agfA 基因在多重 PCR 中的利用为肠炎沙门氏菌菌株提供了有价值的诊断工具。
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