对黑腹果蝇中涉及乙醇脱氢酶基因座的常染色体剂量补偿的分析。
Analysis of autosomal dosage compensation involving the alcohol dehydrogenase locus in Drosophila melanogaster.
作者信息
Birchler J A, Hiebert J C, Paigen K
机构信息
Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
出版信息
Genetics. 1990 Mar;124(3):679-86.
Abstract
An example of autosomal dosage compensation involving the expression of the alcohol dehydrogenase (Adh) locus is described. Flies trisomic for a quarter of the length of the left arm of chromosome two, including Adh, have diploid levels of enzyme activity and alcohol dehydrogenase messenger RNA. Subdivision of the compensating trisomic into smaller ones revealed a region that exerts an inverse regulatory effect on alcohol dehydrogenase activity and messenger RNA levels and a smaller region surrounding the structural gene that exhibits a direct gene dosage response. The two opposing effects are of sufficient magnitude that they cancel when simultaneously present resulting in the observed compensation in the larger aneuploid. An Adh promoter-white structural gene fusion construct is affected by the inverse regulatory region indicating that the effect is mediated through the Adh promoter sequences. The role of autosomal dosage compensation in understanding aneuploid syndromes and karyotype evolution in Drosophila species is discussed.
摘要
描述了一个涉及酒精脱氢酶（Adh）基因座表达的常染色体剂量补偿的例子。对于包括Adh在内的二号染色体左臂四分之一长度三体的果蝇，具有二倍体水平的酶活性和酒精脱氢酶信使RNA。将补偿性三体细分为更小的三体后发现，有一个区域对酒精脱氢酶活性和信使RNA水平发挥反向调节作用，以及一个围绕结构基因的较小区域呈现直接的基因剂量反应。这两种相反的效应强度足以在同时存在时相互抵消，从而在较大的非整倍体中产生观察到的补偿。一个Adh启动子-白色结构基因融合构建体受反向调节区域的影响，表明这种效应是通过Adh启动子序列介导的。讨论了常染色体剂量补偿在理解果蝇物种非整倍体综合征和核型进化中的作用。
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本文引用的文献
1
Modulation of protein levels in chromosomal dosage series of maize: the biochemical basis of aneuploid syndromes.玉米染色体剂量系列中蛋白质水平的调节：非整倍体综合征的生化基础。
Genetics. 1981 Oct;99(2):247-66. doi: 10.1093/genetics/99.2.247.
2
The genetic basis of dosage compensation of alcohol dehydrogenase-1 in maize.玉米醇脱氢酶-1 剂量补偿的遗传基础。
Genetics. 1981 Mar;97(3-4):625-37. doi: 10.1093/genetics/97.3-4.625.
3
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
4
The genetics of a small autosomal region of Drosophila melanogaster, including the structural gene for alcohol dehydrogenase. V. Characterization of X-ray-induced Adh null mutations.黑腹果蝇一个常染色体小区域的遗传学，包括乙醇脱氢酶的结构基因。V. X射线诱导的Adh无效突变的特征分析
Genetics. 1982 Nov;102(3):421-35. doi: 10.1093/genetics/102.3.421.
5
Human beta-globin pre-mRNA synthesized in vitro is accurately spliced in Xenopus oocyte nuclei.在体外合成的人β-珠蛋白前体信使核糖核酸（pre-mRNA）在非洲爪蟾卵母细胞核中能被精确剪接。
Cell. 1983 Mar;32(3):681-94. doi: 10.1016/0092-8674(83)90054-5.
6
The messenger RNA for alcohol dehydrogenase in Drosophila melanogaster differs in its 5' end in different developmental stages.黑腹果蝇中乙醇脱氢酶的信使核糖核酸在不同发育阶段，其5'端有所不同。
Cell. 1983 May;33(1):125-33. doi: 10.1016/0092-8674(83)90341-0.
7
Genomic sequencing.基因组测序
Proc Natl Acad Sci U S A. 1984 Apr;81(7):1991-5. doi: 10.1073/pnas.81.7.1991.
8
Regulation of white locus expression: the structure of mutant alleles at the white locus of Drosophila melanogaster.白色基因座表达的调控：黑腹果蝇白色基因座突变等位基因的结构
Cell. 1982 Sep;30(2):529-41. doi: 10.1016/0092-8674(82)90250-1.
9
Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.变性RNA与转移至硝酸纤维素膜上的小DNA片段的杂交。
Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5. doi: 10.1073/pnas.77.9.5201.
10
A simple, rapid, and sensitive DNA assay procedure.一种简单、快速且灵敏的DNA检测程序。
Anal Biochem. 1980 Mar 1;102(2):344-52. doi: 10.1016/0003-2697(80)90165-7.
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