Kim Tae-Il, Chung Chong-Pyoung, Heo Min-Suk, Park Yoon-Jeong, Rhee Sang-Hoon
Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.
J Periodontal Implant Sci. 2010 Oct;40(5):220-6. doi: 10.5051/jpis.2010.40.5.220. Epub 2010 Oct 31.
This study was performed to evaluate the periodontal wound healing effect of particulate equine bone mineral on canine alveolar bone defects.
Twelve adult male beagle dogs were used as study subjects. The mandibular second and fourth premolars were extracted prior to the experimental surgery, and the extraction sites were allowed to heal for 8 weeks. After periodontal probing, two-walled defects were created at the mesial and distal sides of the mandibular third premolars bilaterally, and the defects were filled with equine particulate bone with collagen membrane or bovine particulate bone with collagen membrane, or collagen membrane alone. The defects without any treatment served as negative controls. After probing depth measurement, animals were sacrificed at 10, 16, and 24 post-surgery weeks for micro-computed tomographic and histomorphometric analysis.
The equine particulate bone-inserted group showed significantly decreased values of probing depth and first bone contact compared to the negative control and collagen membrane alone groups at weeks 10, 16, and 24 (P < 0.05). There were no significant differences in the new cementum length, newly-formed bone area, or newly-formed bone volume between equine particulate bone- and bovine particulate bone-inserted groups, both of which showed significantly increased values compared to the negative control and collagen membrane alone groups (P < 0.05).
Equine particulate bone showed significant differences in probing depth, first bone contact, new cementum length, newly formed bone area, and bone volume fraction values when compared to the negative control and collagen membrane alone groups. There were no significant differences between equine and bovine particulate bone substitutes in these parameters; therefore, we can conclude that equine particulate bone is equivalent to bovine bone for periodontal regeneration.
本研究旨在评估颗粒状马骨矿物质对犬牙槽骨缺损的牙周伤口愈合效果。
选用12只成年雄性比格犬作为研究对象。在实验手术前拔除下颌第二和第四前磨牙，拔牙部位让其愈合8周。牙周探诊后，在下颌第三前磨牙双侧的近中和远中侧制造双壁缺损，缺损处分别填充马颗粒骨与胶原膜、牛颗粒骨与胶原膜或仅填充胶原膜。未作任何处理的缺损作为阴性对照。测量探诊深度后，在术后10、16和24周处死动物，进行微型计算机断层扫描和组织形态计量分析。
在第10、16和24周时，与阴性对照和仅使用胶原膜的组相比，植入马颗粒骨的组探诊深度值和首次骨接触明显降低（P < 0.05）。植入马颗粒骨组和植入牛颗粒骨组之间的新牙骨质长度、新形成骨面积或新形成骨体积没有显著差异，这两组与阴性对照和仅使用胶原膜的组相比均显示出明显增加的值（P < 0.05）。
与阴性对照和仅使用胶原膜的组相比，马颗粒骨在探诊深度、首次骨接触、新牙骨质长度、新形成骨面积和骨体积分数值方面有显著差异。在这些参数上，马颗粒骨和牛颗粒骨替代物之间没有显著差异；因此，我们可以得出结论，马颗粒骨在牙周再生方面等同于牛骨。
