Lee Chang-Kyun, Koo Ki-Tae, Kim Tae-Il, Seol Yang-Jo, Lee Yong-Moo, Rhyu In-Chul, Ku Young, Chung Chong-Pyoung, Park Yoon-Jeong, Lee Jue-Yeon
Department of Periodontology and Dental Research Institute, Seoul National University School of Dentistry, Seoul, Korea.
J Periodontal Implant Sci. 2010 Oct;40(5):232-8. doi: 10.5051/jpis.2010.40.5.232. Epub 2010 Oct 31.
To prolong the degradation time of collagen membranes, various cross-linking techniques have been developed. For cross-linking, chemicals such as formaldehyde and glutaraldehyde are added to collagen membranes, but these chemicals could adversely affect surrounding tissues. The aim of this study is to evaluate the ability of porous non-chemical cross-linking porcine-derived collagen nanofibrous membrane to enhance bone and associated tissue regeneration in one-wall intrabony defects in beagle dogs.
The second and third mandibular premolars and the first molars of 2 adult beagles were extracted bilaterally and the extraction sites were allowed to heal for 10 weeks. One-wall intrabony defects were prepared bilaterally on the mesial and distal side of the fourth mandibular premolars. Among eight defects, four defects were not covered with membrane as controls and the other four defects were covered with membrane as the experimental group. The animals were sacrificed 10 weeks after surgery.
Wound healing was generally uneventful. For all parameters evaluating bone regeneration, the experimental group showed significantly superior results compared to the control. In new bone height (NBh), the experimental group exhibited a greater mean value than the control (3.04 ± 0.23 mm/1.57 ± 0.59, P = 0.003). Also, in new bone area (NBa) and new bone volume (NBv), the experimental group showed superior results compared to the control (NBa, 34.48 ± 10.21% vs. 5.09 ± 5.76%, P = 0.014; and NBv, 28.04 ± 12.96 vs. 1.55 ± 0.57, P = 0.041). On the other hand, for parameters evaluating periodontal tissue regeneration, including junctional epithelium migration and new cementum height, there were no statistically significant differences between two groups.
Within the limitations of this study, this collagen membrane enhanced bone regeneration at one-wall intrabony defects. On the other hand, no influence of this membrane on periodontal tissue regeneration could be ascertained in this study.
为延长胶原膜的降解时间，已开发出多种交联技术。在交联过程中，会向胶原膜中添加甲醛和戊二醛等化学物质，但这些化学物质可能会对周围组织产生不利影响。本研究的目的是评估多孔非化学交联猪源胶原纳米纤维膜在比格犬单壁骨内缺损中促进骨及相关组织再生的能力。
双侧拔除2只成年比格犬的第二和第三下颌前磨牙以及第一磨牙，拔牙部位让其愈合10周。在下颌第四前磨牙的近中和远中双侧制备单壁骨内缺损。在八个缺损中，四个缺损不覆盖膜作为对照，另外四个缺损覆盖膜作为实验组。术后10周处死动物。
伤口愈合总体顺利。在所有评估骨再生的参数方面，实验组的结果明显优于对照组。在新骨高度（NBh）方面，实验组的平均值高于对照组（3.04±0.23毫米/1.57±0.59，P = 0.003）。此外，在新骨面积（NBa）和新骨体积（NBv）方面，实验组的结果也优于对照组（NBa，34.48±10.21%对5.09±5.76%，P = 0.014；NBv，28.04±12.96对1.55±0.57，P = 0.041）。另一方面，在评估牙周组织再生的参数方面，包括结合上皮迁移和新牙骨质高度，两组之间没有统计学上的显著差异。
在本研究的局限性内，这种胶原膜增强了单壁骨内缺损处的骨再生。另一方面，在本研究中未确定该膜对牙周组织再生有影响。
