Mitochondrial Structure and Dynamics Group, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077, Goettingen, Germany,
Adv Biochem Eng Biotechnol. 2011;124:1-19. doi: 10.1007/10_2010_81.
Heterogeneity in the shapes of individual multicellular organisms is a daily experience. Likewise, even a quick glance through the ocular of a light microscope reveals the morphological heterogeneities in genetically identical cultured cells, whereas heterogeneities on the level of the organelles are much less obvious. This short review focuses on intracellular heterogeneities at the example of the mitochondria and their analysis by fluorescence microscopy. The overall mitochondrial shape as well as mitochondrial dynamics can be studied by classical (fluorescence) light microscopy. However, with an organelle diameter generally close to the resolution limit of light, the heterogeneities within mitochondria cannot be resolved with conventional light microscopy. Therefore, we briefly discuss here the potential of subdiffraction light microscopy (nanoscopy) to study inner-mitochondrial heterogeneities.
个体多细胞生物形状的异质性是日常生活中的常见现象。同样，即使快速浏览一下光学显微镜的目镜，也能发现遗传上相同的培养细胞存在形态异质性，而细胞器水平的异质性则不那么明显。本综述以线粒体为例聚焦于细胞内异质性，并探讨通过荧光显微镜对其进行分析。通过经典（荧光）明场显微镜可以研究整体线粒体形状和线粒体动力学。然而，由于细胞器直径通常接近光的分辨率极限，因此无法通过传统的明场显微镜解析线粒体内部的异质性。因此，我们在这里简要讨论了亚衍射光显微镜（纳米显微镜）研究线粒体内部异质性的潜力。
